高乌头活性成分提取工艺的优化

谢斌, 周军辉, 石新卫, 李思锋

doi:10.3724/SP.J.1259.2014.00198

植物学报 >

2014 , Vol. 49 >Issue 2: 198 - 202

DOI: https://doi.org/10.3724/SP.J.1259.2014.00198

技术方法

高乌头活性成分提取工艺的优化

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陕西省西安植物园, 陕西省植物研究所, 西安 710061

收稿日期: 2013-01-10

修回日期: 2013-03-29

网络出版日期: 2014-01-07

基金资助

陕西省科学院科技计划项目

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Optimization of Extracting Technology for the Active Components in Aconitum sinomontanum

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Xi’an Botanical Garden of Shaanxi Province, Institute of Botany of Shaanxi Province, Xi’an 710061, China

Received date: 2013-01-10

Revised date: 2013-03-29

Online published: 2014-01-07

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摘要

为开发并优化高乌头(Aconitum sinomontanum)中有效成分高乌甲素及冉乌头碱的提取工艺, 采用高效液相色谱法测定高乌甲素及冉乌头碱含量, 以二者综合提取率为考察指标, 采用正交实验设计优选最佳提取工艺。结果表明, 优选工艺为药材粉末加10倍量的80%deg;C下回流提取6小时。经验证, 优选所得的工艺稳定可行, 可作为高乌头活性成分提取的新工艺。

关键词： 高乌头; 高乌甲素; 冉乌头碱; 提取工艺; 正交实验; 高效液相色谱法

本文引用格式

谢斌, 周军辉, 石新卫, 李思锋 . 高乌头活性成分提取工艺的优化[J]. 植物学报, 2014 , 49(2) : 198 -202 . DOI: 10.3724/SP.J.1259.2014.00198

Abstract

To develop and optimize the technology to extract lappaconitine and ranaconitine from Aconitum sinomontanum Nakai, we detected comprehensive extraction rates as an index by high-performance liquid chromatography. The orthogonal test was used for optimizing the extraction technology. Lappaconitine and ranaconitine were extracted with 10 times 80% methanol and 2% material amount of HCl added to the material powder, then refluxed for 6 h under 75°C. The optimized technique is stable and can be used for extracting active components from A. sinomontanum.

Key words： Aconitum sinomontanum; lappaconitine; ranaconitine; extracting technology; orthogonal test; HPLC

参考文献

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