快速、无损大豆种子连续取样技术及其DNA制备

夏正俊, 李玉卓, 朱金龙, 吴红艳, 徐坤, 翟红

doi:10.11983/CBB20095

植物学报 >

2021 , Vol. 56 >Issue 1: 56 - 61

DOI: https://doi.org/10.11983/CBB20095

技术方法

快速、无损大豆种子连续取样技术及其DNA制备

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¹中国科学院种子创新研究院东北地理与农业生态研究所, 哈尔滨 150081
²中国科学院大学, 北京 100049

*E-mail: xiazhj@iga.ac.cn

收稿日期: 2020-05-26

录用日期: 2020-10-05

网络出版日期: 2020-10-11

基金资助

中国科学院重点部署项目(ZDRW-ZS-2019-2);中国科学院战略性科技先导专项(A类)(XDA24010105-4);国家自然科学基金(31771869);国家自然科学基金(31771818);国家自然科学基金(31971970)

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A Rapid, Non-destructive and Continuous Sampling Technique and DNA Extraction for Soybean Seed

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¹Northeast Institute of Geography and Agroecology, the Innovative Academy of Seed Design, Chinese Academy of Sciences, Harbin 150081, China
²University of Chinese Academy of Sciences, Beijing 100049, China

Received date: 2020-05-26

Accepted date: 2020-10-05

Online published: 2020-10-11

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摘要

建立简便、快速和无损种子连续取样技术流程及基因型鉴定技术体系, 可节约种植成本及缩短鉴定周期, 提高基因功能研究和育种效率。该研究利用微型电钻和空气泵等简单装置设计了一种连续且无损钻取大豆(Glycine max)种子组织的方法, 并优化了利用384深孔微孔板高通量提取DNA及基因型鉴定技术体系。该方法也可用于水稻(Oryza sativa)和玉米(Zea mays)等多种主要作物的种子取样及基因型鉴定。

关键词： 大豆(Glycine max); 种子; 钻孔法; 连续取样; 384深孔微孔板; 基因型鉴定

本文引用格式

夏正俊, 李玉卓, 朱金龙, 吴红艳, 徐坤, 翟红 . 快速、无损大豆种子连续取样技术及其DNA制备[J]. 植物学报, 2021 , 56(1) : 56 -61 . DOI: 10.11983/CBB20095

Abstract

Establishment of a simple, quick, non-destructive, and continuous sampling procedure, can save planting cost and accelerate gene functional analysis and breeding procedures. In this study, we created a rapid, non-destructive, and continuous sampling technique using micro electric driller and air pump. We also optimized the high throughput DNA extraction method for the 384 deep-well plate and genotyping method. Furthermore, this technique could be applied in rice, maize and other crops for seed sampling and high throughput genotyping.

Key words： soybean (Glycine max); seed; drilling; continuing sampling; 384 deep-well plate; genotyping

参考文献

[1]	程文, 夏正俊, 冯献忠, 杨素欣 (2016). 一种快速、无损大豆种子DNA提取方法的建立和应用. 植物学报 51, 68-73.
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