拟南芥黏连蛋白RAD21对增强UV-B辐射后细胞分裂的响应
收稿日期: 2020-01-15
录用日期: 2020-05-15
网络出版日期: 2020-05-20
基金资助
国家自然科学基金(30671061);山西省研究生教育创新项目(2019SY3131);山西师范大学研究生科技创新项目(01053011)
Response of Arabidopsis Cohesin RAD21 to Cell Division after Enhanced UV-B Radiation
Received date: 2020-01-15
Accepted date: 2020-05-15
Online published: 2020-05-20
UV-B辐射对植物的影响体现在多个水平, 其会引起植物DNA损伤, 造成有丝分裂异常, 最终影响植物的生长发育及生理生化过程。RAD21.3是黏连蛋白复合物的一个亚基, 参与有丝分裂中染色体的分离。该研究以哥伦比亚生态型拟南芥(Arabidopsis thaliana)和atrad21.3突变体为材料, 设置对照(CK)及UV-B处理组, 对野生型(WT)、atrad21.3及过表达株系的根长、株高、抽薹时间和生理生化指标进行统计分析。利用碱性品红染色观察拟南芥根尖的有丝分裂现象, 并统计畸变率。SPSS分析结果表明, UV-B处理后, WT UV-B和atrad21.3 CK的抽薹时间、株高及各项生理生化指标与WT CK相比无显著差异, 但atrad21.3 UV-B与之相比差异显著。通过烟草(Nicotiana benthamiana)的瞬时表达和亚细胞定位观察, 发现RAD21.3集中在细胞核; 进一步观察分裂期细胞发现落后染色体、染色体桥和游离染色体等异常现象。统计结果表明, 与WT CK相比, WT UV-B和atrad21.3 CK的畸变率较高, 但atrad21.3 UV-B的畸变率更高, 表明RAD21.3可能响应UV-B辐射诱导的异常有丝分裂。
关键词： 拟南芥; atrad21.3突变体; UV-B辐射; 染色体; 有丝分裂
贺芳芳,陈慧泽,冯金林,高琳,牛娇,韩榕 . 拟南芥黏连蛋白RAD21对增强UV-B辐射后细胞分裂的响应[J]. 植物学报, 2020 , 55(4) : 407 -420 . DOI: 10.11983/CBB20009
The effect of UV-B radiation on plants is reflected in multiple levels. DNA damage is caused by UV-B radiation, which leads to abnormal mitosis and ultimately affects plant growth and physiological and biochemical processes. RAD21.3 is a subunit of cohesin complex, which is involved in chromosome separation during mitosis. In this paper, Columbia-0 Arabidopsis thaliana and atrad21.3 were used as materials, and the control and UV-B treatment group were set up to analyze the root length, plant height, bolting time, physiological and biochemical parameters of WT, atrad21.3 and overexpressed transgenic plants. The mitosis of Arabidopsis root tip cells was observed by basic fuchsin staining, and the aberration rate was counted. After UV-B treatment of the WT and atrad21.3 mutants, it was found that the UV-B treated-WT and atrad21.3 had similar bolting time, plant height and various physiological and biochemical indexes. Through the construction of expression vector, the results showed that RAD21.3 was located in the nucleus. Further observation of mitosis revealed abnormal phenomena such as lagging chromosomes, chromosomes bridge, fragments chromosomes, etc. Statistics show that the aberration rate of the UV-B treated-WT is similar to atrad21.3, and the aberration rate of the UV-B treated-atrad21.3 increases. The above results indicate that RAD21.3 may respond to abnormal mitosis induced by UV-B radiation.
Key words： Arabidopsis thaliana; atrad21.3 mutant; UV-B radiation; chromosome; mitosis
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