以实生桃(Prunus persica)苗为试材, 探讨SnRK1对不同浓度蔗糖及处理时间的响应特性, 揭示蔗糖对植株生长发育的影响, 以期为果树生产提供理论依据及技术支持。结果表明, 施加5%蔗糖时, 植株体内SnRK1酶活性最高, 且在一定时间内, 酶活性持续升高; 与对照(清水和甘露醇)相比, 5%蔗糖处理显著提高植株可溶性糖、淀粉和叶片叶绿素含量, 增加植株地上部和地下部生物量, 显著加快植株净光合速率; 通过观察根系构型, 发现5%蔗糖可以显著增加根系总表面积、总体积和侧根数量, 并可促进根系加粗加长生长。qRT-PCR分析表明, 外源蔗糖能促进根系中生长素的合成和转运。综上, 一定浓度蔗糖可以提高植株体内SnRK1酶活性, 影响植株碳代谢, 促进植株生长发育, 且增加根系生长素的合成与转运, 进而影响根系构型。

This study analyzed the SnRK1 activity and seedling growth and development in response to different concentrations and durations of exogenous sucrose in peach (Prunus persica), thus providing theoretical basis and technical support for fruit production. The data showed that 5% sucrose treatment greatly enhanced the SnRK1 activity, which was continuously increased within a certain period of treatment. Compared with water and mannitol controls, 5% sucrose treatment significantly increased the contents of soluble sugar, starch, and chlorophyll, the biomass of aboveground and underground, and the net photosynthetic rate. Examination of root configuration showed that 5% sucrose significantly increased root surface area, root volume, and number of lateral roots, and promoted root thickening and elongation. qRT-PCR analysis showed that exogenous sucrose promoted auxin synthesis and transport in roots. In conclusion, application of certain concentration of sucrose promotes SnRK1 activity, carbon metabolism, and plant growth and development, increases root auxin synthesis and transport and affects root configuration in peach.