真空干燥箱已越来越广泛地应用于现代生物学研究领域。该文利用真空干燥箱温度和负压的可控制性能, 将固定、脱水、透明和石蜡渗透等过程在真空干燥箱中进行, 建立起一套可行的植物组织快速石蜡制片方法。结果显示, 真空干燥箱的应用加速了多种试剂的渗透速率, 提高了切片质量, 达到了优化实验步骤、节省实验时间和减少室内有毒化学气体污染的目的。
The vacuum drying oven has been widely used in modern biology research. We developed a fast method for paraffin sectioning of plant tissue using a vaccum drying oven in which temperature and negative pressure was controlled. The fixation, dehydration, transparency, and paraffin infiltration can be performed in the vacuum drying oven. Use of the oven in plant-tissue paraffin sectioning accelerates the infiltration rate, improves the quality of sections and save time. It also reduces the toxic chemical fumes with indoor pollution.
参考文献：
李和平（2009）.植物显微技术.北京:科学出版社.pp.21—28.
贺运春（2002）.植物病害制片技术. 北京:中国农业出版社.pp.54—66.
任成林，王鹏，杨永红（2007）.常规加温、震荡在组织石蜡切片中的比较试验.黑龙江畜牧兽医（7）：94-95.
潘叶，冯永庆，马焕普（2008）.一种适用于植物组织的快速石蜡制片法.中国农学报24（3）：112—115.
魏欣悦，苏源，杨静等 （2011）.微波快速石蜡切片法观察水稻叶片组织.云南农业大学学报26（4）:453—457.
王静，左辛，黄正文（2011）.常规植物石蜡制片技术的改进及应用.安徽农学通报17（11）：36-39.
李正理（1996）.植物组织制片学.北京:北京大学出版社.pp.34-130
周仪（2008）.植物形态解剖实验.北京:北京师范大学出版社.pp.126.
胡正海（2010）.植物解剖学.北京:高等教育出版社.pp.213—223.
乌仁陶格斯，闫伟，韩胜利（2010）.不同石蜡制片法对外生菌根显微结构观察效果的研究.内蒙古农业大学学报31.75.
杨虎彪，李晓霞，罗丽娟（2009）.植物石蜡制片中透明和脱蜡技术的改良.植物学报.44.2：230—235.
Z.Q.Yuan,C.Mohammed(2001)Lesion development in stems of rough and smooth barked Eucalyptus mittens following artificial inoculations with canker fungi.Forest Pathology 31,149-161.
Anik Chevrier,Evgeny Rossomacha ,Michael.D.Buschmann.(2005) Optimization of Histoprocessing Methods to detect Glycosamiinoglycan,collagen typeⅡ,and collagen typeⅠin decalcified rabbit osteochondral sections.The Journal of Histotechnology 28,165-175.
Elmon Schmelzer,Susanne Kruger-Lebus,Klaus Hahlbrock.(1989) Temporal and spatial patterns of gene expresstion around sites of attempted fungal infection in parsley leaves.The Plant Cell October,1993-1001.
Wei Hua Tang,Sean Coughlan,Edmuud Crane,Mary Beatty.(2006)The application of laser microdissection to in planta gene expression profiling of the Maize Anthracnose Stalk Rot fungus Collectotrichum graminicola.Molecular Plant-Microbe Interactions 19.1240-1250.
首页