以海三棱藨草(Scirpus × mariqueter)成熟种子为外植体, 通过无菌萌发、丛生芽诱导、增殖、壮苗、生根和移栽等过程, 建立了海三棱藨草的无菌快繁体系。结果表明: 丛生芽诱导和增殖的最适培养基为MS+2.0 mg·L^-1 6-BA+0.002 mg·L^-1 TDZ+0.2 mg·L^-1 IBA; 壮苗最适培养基为1/2MS+0.05 mg·L^-1 6-BA+0.01 mg·L^-1 IBA; 生根最适培养基为1/2 MS+0.2 mg·L^-1 IBA; 最适培养温度为30°C; 再生苗移入珍珠岩:草炭:蛭石=1:1:1 (体积比)的混合基质中, 移栽成活率可达85%以上。生根培养阶段选用容积较大的塑料容器育苗, 可以降低生产成本和提高生产效率。

The mature seeds of Scirpus × mariqueter were used as explants. Through germ-free germination, induction of multiple shoots, multiplication, rooting and transplantion, an aseptic-frequency regeneration system was established. The optimal medium for inducing adventitious plants and multiplication was MS+2.0 mg·L^-1 6-BA+0.002 mg·L^-1 TDZ+0.2 mg·L^-1 IBA; the optimal medium for culturing strong plants with multiple shoots was 1/2MS+0.05 mg·L^-1 6-BA+0.01 mg·L^-1 IBA; the optimal medium for rooting was 1/2MS+0.2 mg·L^-1 IBA; and the optimal culture temperature was 30°C. Regenerated seedlings were transplanted into the mixture of 3 parts perlite, turf and vermiculite, volume ratio 1:1:1, and the survival rate was >85%. A large-volume plastic container for rooting culture can decrease the cost of production and increase production efficiency.