CRISPR/Cas9系统在植物基因组编辑中技术改进与创新的研究进展
收稿日期: 2018-07-02
录用日期: 2018-08-09
网络出版日期: 2018-12-07
基金资助
国家重点研发计划(2017YFC1702705、山东省现代农业产业技术体系No.SDAIT-20-04和山东省重点研发计划No.2017CXGC1302)
Recent Progress in Evolutionary Technology of CRISPR/Cas9 System for Plant Genome Editing
Received date: 2018-07-02
Accepted date: 2018-08-09
Online published: 2018-12-07
CRISPR/Cas9基因组编辑技术是一项对基因组进行精准修饰的技术, 可实现对靶标基因的碱基插入、缺失或DNA片段替换。随着人们对CRISPR/Cas9系统的了解逐渐加深, 其在科研、农业和医疗等领域的应用也越来越广泛。该文简要介绍了CRISPR/Cas9基因组编辑技术的发展以及工作原理, 总结了近几年对该技术进行优化与改进的研究进展, 包括基因组编辑效率的提升、基因组编辑范围的扩展、单碱基精准编辑以及多基因同时编辑、基因组编辑安全性的提升以及基因片段替换与基因靶向转录调控, 以期为深入开展这一领域的研究提供参考。
关键词： CRISPR/Cas9; 基因组编辑; 遗传改良; 植物基因组
苏钺凯,邱镜仁,张晗,宋振巧,王建华 . CRISPR/Cas9系统在植物基因组编辑中技术改进与创新的研究进展[J]. 植物学报, 2019 , 54(3) : 385 -395 . DOI: 10.11983/CBB18151
CRISPR/Cas9 genome editing is used for precisely modifying the genome, enabling nucleotide insertion, deletion, or DNA fragment replacement in targeted gene(s). With more understanding of the CRISPR/Cas9 system, this technology has been widely applied in research, agriculture, medical treatment and other fields. This article briefly introduces the discovery and working principle of the CRISPR/Cas9 genome-editing technology, and summarizes the research progress in optimizing and improving the technology in recent years, including improving the gene editing efficiency, the range expansion of gene editing, base editing and multigene editing, the safety enhancement of genome editing, replacing gene fragments and transcriptional regulation of targeted genes, to provide references for thorough research work in this area.
Key words： CRISPR/Cas9; genome editing; genetic improvement; plant genomes
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