异源过表达水稻OsSAPP3基因促进拟南芥叶片衰老
收稿日期: 2018-02-12
录用日期: 2018-05-21
网络出版日期: 2019-07-31
基金资助
国家自然科学基金(31401298)
Heterologous Overexpression of Rice OsSAPP3 Gene Promotes Leaf Senescence in Transgenic Arabidopsis
Received date: 2018-02-12
Accepted date: 2018-05-21
Online published: 2019-07-31
蛋白磷酸酶催化的蛋白质可逆磷酸化反应是叶片衰老的关键环节。该研究筛选并克隆了1个新的参与水稻(Oryza sativa)叶片衰老调控的PP2C基因OsSAPP3。研究表明, OsSAPP3的启动子在ProOsSAPP3-GUS转基因拟南芥(Arabidopsis thaliana)的莲座叶中有活性, 并且活性以依赖叶龄方式增加。利用CaMV 35S启动子驱动组成型异源过表达OsSAPP3导致转基因拟南芥无法正常生长。用可诱导型启动子GVG系统驱动OsSAPP3异源过表达导致转基因拟南芥出现莲座叶变小、数量增加、叶片早衰及抽薹开花提前等早衰表型。外源诱导OsSAPP3基因异源过表达后, 利用实时荧光定量PCR检测到SAG12、WRKY6和NAC2等衰老标志基因显著上调表达。研究结果表明, OsSAPP3是参与水稻叶片衰老的正向调控因子。
崔胜男,张艺函,徐凡 . 异源过表达水稻OsSAPP3基因促进拟南芥叶片衰老[J]. 植物学报, 2019 , 54(1) : 46 -57 . DOI: 10.11983/CBB18045
Protein phosphatase-catalyzed reversible phosphorylation of proteins is a key aspect of leaf senescence. We screened and cloned a new PP2C gene, OsSAPP3, involved in leaf senescence regulation in rice. The promoter of OsSAPP3 was active in rosette leaves of ProOsSAPP3-GUS transgenic Arabidopsis and its activity increased with leaf age. With heterologous overexpression of OsSAPP3 driven by a CaMV 35S promoter, transgenic Arabidopsis could not grow normally. Therefore, OsSAPP3 heterologous overexpression was induced by the inducible promoter GVG system. Exo- genous inducible heterologous overexpression of OsSAPP3 led to a precocious leaf senescence phenotype in transgenic Arabidopsis including smaller rosette leaves and increased number, premature leaf senescence, and advanced bolting and flowering. Additionally, quantitative RT-PCR showed that exogenous inducible heterologous overexpression of OsSAPP3 upregulated the expression of critical senescence-related transcription factor genes, including SAG12, WRKY6, and NAC2. In summary, OsSAPP3 is a positive factor involved in regulating leaf senescence in rice.
Key words： Arabidopsis; leaf senescence; OsSAPP3; 2C type protein phosphatase
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