谷子bZIP转录因子的全基因组鉴定及其在干旱和盐胁迫下的表达分析
# 共同第一作者
收稿日期: 2015-08-18
录用日期: 2016-03-25
网络出版日期: 2018-08-09
基金资助
国家948项目(No.2014-Z39)、山西省煤基重点科技攻关项目(No.FT-2014-01)、山西省回国留学人员科研基金(No.2015-064)和国家自然科学基金(No.31401430, No.31201266, No.31501323)
Genome-wide Characterization of bZIP Transcription Factors in Foxtail Millet and Their Expression Profiles in Response to Drought and Salt Stresses
# Co-first authors
Received date: 2015-08-18
Accepted date: 2016-03-25
Online published: 2018-08-09
bZIP蛋白是植物转录因子中最大和最保守的一类转录因子, 参与调控植物生长发育等多种生命活动。谷子(Setaria italica)是一种重要的C4杂粮作物, 其bZIP基因家族与功能报道较少。利用生物信息学工具, 从谷子全基因组中鉴定出73个SibZIP转录因子, 划分为A、B、C、D、E、G、H、I和X等亚家族。与已测序的禾谷类作物相比, 谷子SibZIP基因家族在进化中发生缩减。在谷子SibZIP蛋白中检测到25种不同的保守氨基酸基序。RNA-seq和定量PCR检测结果表明, 在干旱和盐胁迫条件下, 多数SibZIPs基因不同程度地被诱导表达, 预示着部分SibZIP成员在谷子干旱和盐胁迫响应中起重要作用。共表达关联性分析进一步揭示19个谷子SibZIP转录因子可通过与蛋白激酶或NPR1相关调节蛋白等互作介导谷子胁迫响应。研究结果为全面解析谷子SibZIPs基因结构与生物学功能、抗旱分子机制以及分子育种提供了新信息。
刘宝玲, 张莉, 孙岩, 薛金爱, 高昌勇, 苑丽霞, 王计平, 贾小云, 李润植 . 谷子bZIP转录因子的全基因组鉴定及其在干旱和盐胁迫下的表达分析[J]. 植物学报, 2016 , 51(4) : 473 -487 . DOI: 10.11983/CBB15148
The bZIP protein family is one of the largest and most conserved transcription factor families regulating multiple physiology processes in plants. Foxtail millet (Setaria italica) is an important C4 cereal crop with increased resistance to drought stress. However, little is known about bZIP family members and their functions in this crop. In the present study, we identified and characterized 73 SibZIP transcription factors in foxtail millet genome by using bioinformatics. These SibZIPs are classified into 9 groups, including A, B, C, D, E, G, H, I and X subfamilies. Compared to the sequenced cereal crops, the foxtail millet bZIP family underwent contraction in evolution. In all, 25 conserved motifs were detected among SibZIPs. RNA-seq and qPCR analysis revealed that a number of SibZIPs are induced to change expression levels in response to drought and salt stresses, which suggests that they have important functions in the foxtail millet response to stress. Moreover, correlation analysis of co-expression of the protein coding genes under various stress conditions demonstrates that a set of 19 SibZIPs may mediate the regulation network controlling stress responses by cooperating with some protein kinases or NPR1-related regulatory protein in foxtail millet. Our results could provide new valuable information for comprehensively understanding SibZIP protein structure and their biological functions, the molecular mechanism of drought response, and stress resistance breeding in foxtail millet and related crops.
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