技术方法

新疆阿魏特征显微结构的三维原位无损研究

展开
  • 1新疆医科大学医学工程技术学院, 乌鲁木齐 830011
    2新疆维吾尔自治区中药民族药研究所, 乌鲁木齐 830002
    3新疆医科大学中医学院, 乌鲁木齐 830011
    4乌鲁木齐市中医医院, 乌鲁木齐 830000

收稿日期: 2017-05-23

  录用日期: 2017-10-07

  网络出版日期: 2018-09-11

3-D In-situ Non-destructive Structural Characterization of Ferula sinkiangensis

Expand
  • 1College of Medical Engineering and Technology, Xinjiang Medical University, Urumqi 830011, China
    2Xinjiang Uygur Autonomous Region Institute of Traditional Chinese Medicine and Ethno-Medicine, Urumqi 830002, China
    3College of Chinese Medicine, Xinjiang Medical University, Urumqi 830011, China
    4Urumqi Hospital of Traditional Chinese Medicine, Urumqi 830000, China

Received date: 2017-05-23

  Accepted date: 2017-10-07

  Online published: 2018-09-11

摘要

利用新型第三代高亮度同步辐射光源相衬成像技术, 可实现弱吸收(主要含碳、氢、氧和氮等元素)物质材料特征结构的无损三维鉴定。以具有极高药用价值的新疆地产药材——阿魏(Ferula)为研究对象, 采用同步辐射高分辨X射线相衬显微CT技术, 并结合相位恢复算法, 有效解析和评价了新疆阿魏(F. sinkiangensis)的三维特征结构, 极大提高了对药材类生物样品的显微结构和密度的分辨能力, 从而为新疆阿魏的特征分辨、品质鉴定和真伪识别提供了一种直观可靠的三维可视化新手段。

本文引用格式

刘慧强, 凯撒·苏来曼, 孙芸, 庞渊, 樊孝喜, 谢茹, 柳超, 段颖妮, 马燕 . 新疆阿魏特征显微结构的三维原位无损研究[J]. 植物学报, 2018 , 53(3) : 364 -371 . DOI: 10.11983/CBB17104

Abstract

Synchrotron-based X-ray phase-contrast micro-tomography is being used for achieving nondestructive and 3-D characterization due to the high contrast imaging of low Z materials (consisting of C, H, O, N elements). In this paper, we present a new method to combine the high resolution synchrotron-based X-ray phase-contrast imaging technology and phase retrieval algorithm for analyzing and evaluating the 3D inner micro-structures and nondestructive characteristic structures of Ferula sinkiangensis. The method successfully revealed the 3-D micro-structures and characteristics of F. sinkiangensis with high-density resolution, demonstrating that the method is an intuitive and reliable tool of 3D visualization, which has good potential for characterizing and identifying Chinese medicine materials.

参考文献

[1] 黄博, 姜兆玉, 屈红霞, 马三梅 (2010). 龙牙花不同花器官的表皮形态. 植物学报 45, 594-603.
[2] 孔妤, 王忠, 顾蕴洁, 汪月霞 (2008). 植物根内通气组织形成的研究进展. 植物学通报 25, 248-253.
[3] 黎耀东, 付淑媛, 何江, 樊丛照, 李晓瑾 (2016). 新疆特有药用植物新疆阿魏资源现状与分析. 中国现代中药 18, 714-718.
[4] 刘慧强, 任玉琦, 周光照, 和友, 薛艳玲, 肖体乔 (2012). 相移吸收二元性算法用于X射线混合衬度定量显微CT的可行性研究. 物理学报 61, 078701.
[5] 刘慧强, 王玉丹, 任玉琦, 薛艳玲, 和友, 郭瀚, 肖体乔 (2012). 采用吸收修正Bronnikov算法的有机复合样品的X射线显微计算机层析研究. 光学学报 32, 0434001.
[6] 刘家熙, 阎秀峰 (2005). 西藏产四种卷柏科植物的孢子形态观察. 植物学通报 22, 44-49.
[7] 肖体乔, 谢红兰, 邓彪, 杜国浩, 陈荣昌 (2014). 上海光源X射线成像及其应用研究进展. 光学学报 34, 0100001.
[8] 徐晓琴, 倪慧, 魏鸿雁, 贾晓光, 张本刚, 卿德刚 (2013). 新疆地产三种肉苁蓉的显微鉴别研究. 时珍国医国药 24, 881-883.
[9] 薛艳玲, 肖体乔, 吴立宏, 陈灿, 郭荣怡, 杜国浩, 谢红兰, 邓彪, 任玉琦, 徐洪杰 (2010). 利用X射线相衬显微研究野山参的特征结构. 物理学报 59, 5496-5507.
[10] 叶琳琳, 薛艳玲, 倪梁红, 肖体乔 (2014). 种子类中药材的三维显微结构的原位研究. 中国中药杂志 39, 2619-2623.
[11] 叶琳琳, 薛艳玲, 谭海, 陈荣昌, 戚俊成, 肖体乔 (2013). X射线相衬显微层析及其在野山参特征结构的定量三维成像研究. 光学学报 33, 1234002.
[12] Paganin D, Mayo SC, Gureyev TE, Miller PR, Wilkins SW (2002). Simultaneous phase and amplitude extraction from a single defocused image of a homogeneous object.J Microscopy 206, 33-40.
[13] Xie HL, Deng B, Du GH, Fu YN, Chen RC, Zhou GZ, Ren YQ, Wang YD, Xue YL, Peng GY, He Y, Guo H, Xiao TQ (2015). Latest advances of X-ray imaging and biomedical applications beamline at SSRF.Nucl Sci Tech 26, 020102.
[14] Xue Y, Liang Z, Tan H, Ni L, Zhao Z, Xiao T, Xu H (2016). Microscopic identification of Chinese medicinal materials based on X-ray phase contrast imaging: from qualitative to quantitative.J Instrum 11, C07001.
[15] Ye LL, Xue YL, Ni LH, Tan H, Wang YD, Xiao TQ (2013). Application of X-ray phase contrast micro-tomography to the identification of traditional Chinese medicines.J Ins- trum 8, C07006.
[16] Ye LL, Xue YL, Wang YD, Qi JC, Xiao TQ (2016). Identification of ginseng root using quantitative X-ray microtomography.J Ginseng Res 41, 290-297.
文章导航

/

674-3466/bottom_cn.htm"-->