基于SSR分子标记的酸浆属植物亲缘关系研究
收稿日期: 2018-01-28
录用日期: 2018-05-09
网络出版日期: 2018-06-05
基金资助
国家自然科学基金(No.31470407)、浙江省科技计划(No.2014C32090)和杭州市科技局项目(No.20150932H04, No.20150932H03)
Genetic Relationship of Physalis Plants Revealed by Simple Sequence Repeat Markers
Received date: 2018-01-28
Accepted date: 2018-05-09
Online published: 2018-06-05
近年来, 由于营养价值高、果实可当水果食用且具有潜在的药用价值, 酸浆属(Physalis)植物在全球范围内受到了越来越多的关注。采用SSR分子标记技术对我国范围内主要分布的4种酸浆属植物的22份样品进行了亲缘关系研究, 结果表明, 20对SSR引物共扩增出118个位点, 其中107个(90.7%)为多态性位点; 平均种间遗传相似系数为0.501。UPGMA聚类和PCoA分析结果得出相似结论, 并且将供试酸浆属植物样品分为两大类。其中, 酸浆(P. alkekengi var. francheti)的所有样品与其它酸浆属植物的遗传距离最远, 单独聚为一类, 与前人的研究结果非常吻合。研究表明, SSR标记遗传信息丰富, 可以用于酸浆属植物的亲缘关系分析。研究结果为酸浆属种质资源保护提供了有效信息, 并且为酸浆属植物的分子辅助育种奠定了重要基础。
朱宇佳, 焦凯丽, 罗秀俊, 冯尚国, 王慧中 . 基于SSR分子标记的酸浆属植物亲缘关系研究[J]. 植物学报, 2018 , 53(3) : 305 -312 . DOI: 10.11983/CBB18027
In recent years, Physalis plants have attracted increasing attention worldwide due to their high nutritional value, edible fruit, and potential medicinal value. In this study, simple sequence repeat (SSR) markers were used to assess genetic relationships with 22 samples of four Physalis species mainly distributed in China. Twenty SSR primer pairs produced 118 loci, 90.7% (107) of which showed polymorphism. The average interspecies similarity coefficient was 0.501, which indicates a degree of genetic relationship among Physalis species. The results of UPGMA dendrography and PCoA plotting were similar, and all Physalis samples were grouped into two clusters. All P. alkekengi var. francheti samples, distant from any other Physalis species, constituted a separate cluster, which confirmed findings of previous studies. This study also indicated that SSR markers are rich in genetic information and could be used to assess the genetic diversity of Physalis species which provides rich useful information for protecting the Physalis germplasm resource and an important foundation for molecular assisted-breeding programs with Physalis.
Key words： Physalis; genetic relationship; SSR markers
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