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植物学报 >

2018 , Vol. 53 >Issue 1: 94 - 103

DOI: https://doi.org/10.11983/CBB16247

研究报告

鲁桑叶绿体基因组序列及特征分析

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  • 西北农林科技大学林学院, 杨凌 712100

收稿日期: 2016-12-13

  录用日期: 2017-03-29

  网络出版日期: 2017-03-29

基金资助

西北农林科技大学唐仲英育种基金(No.2013-14)

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Complete Chloroplast Genome Sequence and Characteristics Analysis of Morus multicaulis

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  • College of Forestry, Northwest A & F University, Yangling 712100, China

Received date: 2016-12-13

  Accepted date: 2017-03-29

  Online published: 2017-03-29

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摘要

鲁桑(Morus multicaulis)是亚洲地区栽培的重要经济作物。以鲁桑品种日本胡橙为实验材料, 利用高通量测序技术对鲁桑叶绿体基因组进行测序, 获得NCBI登录号(KU355297), 并研究鲁桑的叶绿体基因组结构。结合前人对蒙桑(M. mongolica)、印度桑(M. indica)和川桑(M. notabilis)的研究结果, 对鲁桑的系统进化关系进行了探讨。研究结果表明: 鲁桑叶绿体基因组是一个典型的四部分结构, 全长159 154 bp, 共注释130个基因, 包含85个蛋白质编码基因(18个基因在反向重复区重复)、37个转运RNA (tRNA)基因和8个核糖体RNA (rRNA)基因。生物信息学分析表明, 在鲁桑中共搜索到82个SSR位点, 单核苷酸、二核苷酸、三核苷酸、四核苷酸和五核苷酸重复基序个数分别为63、7、2、9和1个, 并没有发现六核苷酸; 其中单核苷酸重复在鲁桑的叶绿体基因组SSR中占76.8%。采用MEGA 6.0软件, 通过最大似然法和近邻结合法对包括4个桑属物种在内的15个物种的叶绿体基因组序列进行聚类分析, 2种方法得到的聚类结果均为鲁桑和蒙桑聚在一起。研究结果对叶绿体基因组工程研究及桑属种间的分子标记开发和优良品种培育具有一定的参考价值。

关键词： 鲁桑; 叶绿体基因组; 高通量测序; 聚类分析

本文引用格式

李巧丽, 延娜, 宋琼, 郭军战 . 鲁桑叶绿体基因组序列及特征分析[J]. 植物学报, 2018 , 53(1) : 94 -103 . DOI: 10.11983/CBB16247

Abstract

Mulberry is an economically important crop in Asia. We determined the complete chloroplast sequence of cultivated species of Morus multicaulis. Ribenhuchen was used as experimental material. High-throughput sequencing was used to sequence the chloroplast genome and the genome structure (NCBI No.: KU355297), and we compared the chloroplast genome with those of reported sibling species (Morus mongolica, M. indica, M. notabilis). The chloroplast genome (cpDNA) of M. multicaulis with a typical quadripartite structure is 159 154 bp long. The cpDNA of M. multicaulis contains 130 genes, including 85 protein coding genes (18 genes duplicated in the inverted repeat regions), 37 transfer RNA genes and 8 ribosomal RNA genes. There are 82 simple sequence repeats, and the number of mono-, di-, tri-, tetra-, pentanucleotide repeat motifs is 63, 7, 2, 9, and 1, with no hexanucleotide repeat sequences. Mono-nucleotide repeat sequences accounted for 76.8% of the cpDNA of simple sequence repeats. MEGA 6.0 was used to construct the phylogenetic tree of 15 species and for cluster analysis of Morus plants. M. multicaulis and M. mongolica were clustered into one group. The research results have reference value for chloroplast genome research, molecular marker development and breeding of mulberry.

Key words： Morus multicaulis; chloroplast genome; high-throughput sequencing; cluster analysis

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