水稻穗发育功能基因对穗期氮肥的响应
# 共同第一作者
收稿日期: 2015-08-03
录用日期: 2015-11-05
网络出版日期: 2018-08-09
基金资助
国家自然科学基金(No.31401324)
Functional Genes of Panicle Development in Response to Nitrogen Fertilizer in Rice
# Co-first authors
Received date: 2015-08-03
Accepted date: 2015-11-05
Online published: 2018-08-09
为探讨氮肥对水稻(Oryza sativa)穗发育的调控作用, 使用高通量测序技术检测氮肥处理前后水稻叶片和幼穗组织中转录组的变化, 并从中筛选到大量差异表达基因。这些基因的功能涉及转录调控、激素代谢和信号转导、物质代谢和转运、胁迫响应、信号转导(受体)和蛋白质降解等。同时对目前克隆得到的穗发育相关基因进行分析, 发现在氮素穗肥的作用下, 部分重要功能基因的表达量发生了明显变化, 其中一些基因还参与调控水稻株高、抽穗期、分蘖和结实率等性状。对这些差异表达基因的功能研究有助于揭示氮素穗肥调控水稻每穗颖花数的分子机制。
丁承强, 王绍华, 丁艳锋 . 水稻穗发育功能基因对穗期氮肥的响应[J]. 植物学报, 2016 , 51(4) : 488 -495 . DOI: 10.11983/CBB15136
Deep-sequencing technologies provide quantitative measures of transcript abundance. We studied the effect of nitrogen fertilizer on genes by deep-sequencing. Many genes were differentially expressed under different levels of nitrogen. The differentially expressed genes were classified into several functional categories including transcriptional regulation, hormone metabolism and signal transduction, metabolism and transportation, stress response, signal transduction (receptor), and protein degradation. Mutant analyses and map-based cloning have revealed many genes controlling the number of spikelets per panicle. Several genes were regulated by nitrogen fertilizer. Some genes regulate panicle development and also plant height, heading date, tiller number, and seed setting rate. Researching the function of these differentially expressed genes will help reveal the molecular mechanisms of nitrogen fertilizer in regulating panicle development in rice.
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