以耐盐植物藜(Chenopodium album)为材料, 利用同源克隆技术获得了7个CDPK基因核心序列, 并将其命名为CaCPK1–7。随后通过RACE技术成功获得CaCPK1–3的开放阅读框(ORF)序列, 其ORF分别包含长度为1 632、1 704和1 590 bp的核苷酸序列。CaCPK1–3分别编码由543、567和529个氨基酸残基组成的钙依赖型蛋白激酶。定量PCR实验显示, CaCPK1–4受盐胁迫诱导明显上调表达, 随胁迫时间增加不同基因呈现各异的表达规律。对CaCPK1–3在其它非生物胁迫下的表达分析显示, CaCPK1、CaCPK2和CaCPK3的表达均受外源ABA和H₂O₂的调控, H₂O₂合成抑制剂DPI和ABA合成抑制剂Na₂WO₄显著抑制300 mmol·L^–1NaCl处理下CaCPK1、CaCPK2和CaCPK3的表达。研究结果为揭示藜在盐胁迫信号转导过程中CDPK基因家族的功能提供了理论依据。

By homology cloning, we obtained the core sequences of 7 calcium-dependent protein kinase (CDPK) genes, CaCPK1 to CaCPK7, from salt-tolerant Chenopodium album. Quantitative real-time PCR revealed that CaCPK1, CaCPK2, CaCPK3 and CaCPK4 were significantly induced by salt stress with different patterns. The expression pattern of CaCPK1, CaCPK2 and CaCPK3 was also changed in C. album under both H₂O₂ and abscisic acid (ABA) stresses. Diphenylene iodonium (DPI, inhibitor of H₂O₂ synthesis) and sodium tungstate (Na₂WO₄, inhibitor of ABA synthesis) significantly inhibited the expression of CaCPK1, CaCPK2 and CaCPK3 on exposure to 300 mmol·L^–1NaCl. We obtained the full-length sequences of 3 CDPKs―CaCPK1, CaCPK2 and CaCPK3―by 3′- and 5′-RACE, which contain open reading frames of 1 632, 1 704, and 1 590 bp, respectively. The production of ABA and H₂O₂ induced by salt may be involved in induction of CaCPK1, CaCPK2 and CaCPK3, facilitating the analysis of the function of CDPK gene family in C. album.