通过构建重组表达质粒载体p139035S-KGF1和根癌农杆菌介导在红花(Carthamus tinctorius)中表达角质细胞生长因子(KGF-1)。从侵染到诱导生根共需要14周, 转化率达0.1%。红花子叶在潮霉素筛选培养基上培养4–5周后便可获得丛生芽, 再生芽移入含潮霉素的伸长生根培养基, 培养4–8周可诱导生根。通过PCR、Southern blot、RT-PCR及Western blot检测证明目的基因KGF-1已经整合到红花细胞的染色体中, 实现了KGF-1外源蛋白在红花中的成功表达, 为开发KGF-1蛋白新的生产途径奠定了基础。

The recombinant plasmid vector p139035S-KGF1 was constructed and transfected into safflower by Agrobacterium tumefaciens transfection. The period of infection to rooting was 14 weeks, and transformation efficiency was about 0.1%. Adventitious shoots were generated after cotyledons were cultured in selecting media containing hygromycin for 4–5 weeks. Rooted safflower was obtained in 4–8 weeks. KGF-1 was transformed into safflower chromosomes and expressed successfully, as determined by PCR, Southern blot, RT-PCR and Western blot analysis, which established the basis for the production of KGF-1.