珍稀濒危植物珙桐胚的萌发与快速繁殖

余阿梅;苏智先*;王立强;方志荣;邹利娟

doi:10.3969/j.issn.1674-3466.2009.04.011

植物学报 >

2009 , Vol. 44 >Issue 04: 491 - 496

DOI: https://doi.org/10.3969/j.issn.1674-3466.2009.04.011

技术方法

珍稀濒危植物珙桐胚的萌发与快速繁殖

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1 西华师范大学环境科学与生物多样性保护重点实验室, 南充 637002; 2 绵阳师范学院, 绵阳 621000; 3 西华师范大学生命科学学院, 南充 637002; 4 西昌学院生化系, 西昌 615003

收稿日期: 2008-07-28

修回日期: 2008-11-24

网络出版日期: 2009-07-01

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Embryo Germination and Rapid Propagation of Rare and Endangered Plant Davidia involucrata

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1Key Laboratory of Environmental and Biodiversity Conservation, China West Normal University, Nanchong 637002, China2Mianyang Normal College, Mianyang 621000, China

Received date: 2008-07-28

Revised date: 2008-11-24

Online published: 2009-07-01

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摘要

首次采用均匀设计和回归分析方法对影响珙桐(Davidia involucrata)胚萌发的3种因素(基本培养基、赤霉素和活性炭)进行优化, 并利用萌发胚建立了珙桐的高效再生体系。利用回归方程得出胚萌发的最优条件为: 在含有0.5 mg.L^{- 1} IBA和1 mg.L^-1 6-BA的1/2MS培养基中添加2.85 mg.L^-1 GA3和0.25 g.L^-1活性炭。在验证实验中珙桐胚萌发率为87.72%, 与预测值无显著差异。最优的芽诱导与增殖条件为: 添加0.1 mg.L^-1 NAA和2.0 mg.L^-1 6-BA 的WPM培养基, 增殖系数为4.34。在生根培养基中增殖的芽生根率高达90%, 移栽成活率为71%。

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余阿梅;苏智先*;王立强;方志荣;邹利娟 . 珍稀濒危植物珙桐胚的萌发与快速繁殖[J]. 植物学报, 2009 , 44(04) : 491 -496 . DOI: 10.3969/j.issn.1674-3466.2009.04.011

Abstract

Three factors (media type, gibberellic acid [GA₃] and active carbon) affecting the embryo germination of Davidia involucrata were optimized by use of a uniform design and analyzed by regression analysis . Then a system for micropropagating D. involucrata was established from the embryos . From regression equations, the optimal conditions for embryo germination were the addition of 2.85 mg.L^-1 GA₃ and 0.25 g.L^-1 activated carbon to 1/2 MS medium containing 0.5 mg.L^-1 IBA and 1 mg.L^-1 6-BA. The germination rate of embryo was 87.72%, without significant deviation to the predicted value. The optimal conditions for shoot induction and multiplication were WPM medium supplemented with 0.1 mg.L^-1 NAA and 2.0 mg.L^-1 6-BA; the mean shoots obtained per explant was 4.34. An amount of 90% of shoots rooted on rooting medium, and 71% plants survived after transplanting.

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