建立盐生植物海马齿(Sesuvium portulacastrum)的离体再生体系,为其生物技术改良奠定基础。以海马齿叶片、茎和腋芽为外植体, 在不同激素配比的培养基上进行愈伤组织诱导、继代培养以及不定芽的分化和生根培养。结果表明: 最适愈伤组织诱导的外植体为叶片, 其次为幼嫩的茎段和腋芽。以叶片为外植体, 愈伤组织诱导率最高的培养基为MS+2.0mg·L–12, 4-D + 0.5 mg·L–16-BA + 3%sucrose; 芽分化最适培养基为MS + 1.0 mg·L–1 2, 4-D + 0.2 mg·L–1 6-BA + 3% sucrose;生根最适培养基为MS + 3%sucrose + 0.1%AC。炼苗移栽后, 成活率可达80%。
We aimed to develop a micropropagation protocol for regenerating tissue in halophilous Sesuvium portulacastrum and improving its basis for biotechnology study. The leaves, shoots and axillary buds were used as explants to induce calli, shoots and roots on different media. The highest efficiency of callus induction was from leaf disc, then stem and axillary bud. The optimal media for inducing calli, shoots and roots from leaves was a base of MS+3% sucrose with 2.0 mg·L–12, 4-D+0.5 mg·L–16-BA; 1.0 mg·L–12, 4-D+0.2 mg·L–16-BA; and 0.1% active carbon, respectively. The survival rate of the cultured seedlings was up to 80% on soil.
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