技术方法

麻竹花药培养及再生植株的获得

展开
  • 1中国林业科学研究院亚热带林业研究所, 富阳 311400; 2中国水稻研究所, 杭州 310006

收稿日期: 2009-01-21

  修回日期: 2009-03-31

  网络出版日期: 2010-01-01

Anther Culture and Plant Regeneration of Dendrocalamus latiflorus

Expand
  • 1Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, China
    2State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China

Received date: 2009-01-21

  Revised date: 2009-03-31

  Online published: 2010-01-01

摘要

以麻竹(Dendrocalamus latiflorus Munro)花药为材料, 于M8+2 mg·L–1 NAA +0.5 mg·L–1 6-BA+15 mg·L–1 PAA+7.5 mg·L–1 STS+500 mg·L–1 CH+100 mg·L–1 proline+100 mg·L–1 glutamin+5.4% maltose+0.8% agar的诱导培养基上成功诱导出胚性愈伤组织, 在此培养基上继代可形成体胚并分化成苗, 初步建立了麻竹花药一步成苗的再生体系。

本文引用格式

乔桂荣;李海营;蒋晶;孙宗修;卓仁英* . 麻竹花药培养及再生植株的获得[J]. 植物学报, 2010 , 45(01) : 88 -90 . DOI: 10.3969/j.issn.1674-3466.2010.01.012

Abstract

Embryogenic callus was initiated from bamboo (Dendrocalamus latiflorus Munro) anthers cultured on M8 medium supplemented with 2 mg·L–1 NAA, 0.5 mg·L–1 6-BA, 15 mg·L–1 phenylacetic acid (PAA), 7.5 mg·L–1 silver thiosulfate (STS), 500 mg·L–1 casein enzymatic hydrolysate (CH), 100 mg·L–1 proline, 100 mg·L–1 glutamine, 5.4% maltose, and 0.8% agar. Subculture of these embryogenic calli on the same medium resulted in embryoid formation and their subsequent germination to form rooted plantlets. A one-step method for anther culture and plant regeneration of D. latiflorus was preliminarily established.

文章导航

/

674-3466/bottom_cn.htm"-->