麻竹花药培养及再生植株的获得
收稿日期: 2009-01-21
修回日期: 2009-03-31
网络出版日期: 2010-01-01
Anther Culture and Plant Regeneration of Dendrocalamus latiflorus
Received date: 2009-01-21
Revised date: 2009-03-31
Online published: 2010-01-01
以麻竹(Dendrocalamus latiflorus Munro)花药为材料, 于M8+2 mg·L–1 NAA +0.5 mg·L–1 6-BA+15 mg·L–1 PAA+7.5 mg·L–1 STS+500 mg·L–1 CH+100 mg·L–1 proline+100 mg·L–1 glutamin+5.4% maltose+0.8% agar的诱导培养基上成功诱导出胚性愈伤组织, 在此培养基上继代可形成体胚并分化成苗, 初步建立了麻竹花药一步成苗的再生体系。
乔桂荣;李海营;蒋晶;孙宗修;卓仁英* . 麻竹花药培养及再生植株的获得[J]. 植物学报, 2010 , 45(01) : 88 -90 . DOI: 10.3969/j.issn.1674-3466.2010.01.012
Embryogenic callus was initiated from bamboo (Dendrocalamus latiflorus Munro) anthers cultured on M8 medium supplemented with 2 mg·L–1 NAA, 0.5 mg·L–1 6-BA, 15 mg·L–1 phenylacetic acid (PAA), 7.5 mg·L–1 silver thiosulfate (STS), 500 mg·L–1 casein enzymatic hydrolysate (CH), 100 mg·L–1 proline, 100 mg·L–1 glutamine, 5.4% maltose, and 0.8% agar. Subculture of these embryogenic calli on the same medium resulted in embryoid formation and their subsequent germination to form rooted plantlets. A one-step method for anther culture and plant regeneration of D. latiflorus was preliminarily established.
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