研究论文

调控类黄酮合成的牡丹R2R3-MYB转录因子鉴定与分析

  • 符真珠 ,
  • 袁欣 ,
  • 高杰 ,
  • 王二强 ,
  • 王晓晖 ,
  • 李艳敏 ,
  • 王慧娟 ,
  • 王亮生 ,
  • 李珊珊 ,
  • 张和臣
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  • 1河南省农业科学院园艺研究所,河南 郑州 450002;2洛阳市农林科学院,河南 洛阳 471026;3中国科学院植物研究所,北京 100093

收稿日期: 2025-08-11

  修回日期: 2025-11-14

  网络出版日期: 2025-12-08

基金资助

国家自然科学基金 (No.32030095)、河南农科学院创新团队(No.2024TD36)、河南省联合基金(No.242301420128)和中央引导地方科技发展资金(No.Z20241471128)

Identification and Analysis of R2R3-MYB Transcription Factors Involved in Flavonoid Biosynthesis in Tree Peony

  • FU Zhen-Zhu ,
  • YUAN Xin ,
  • GAO Jie ,
  • YU Er-Jiang ,
  • YU Xiao-Hui ,
  • LI Yan-Min ,
  • YU Hui-Juan ,
  • YU Liang-Sheng ,
  • LI Shan-Shan ,
  • ZHANG He-Chen
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  • 1 Horticultural Research Institute of Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; 2 Luoyang Academy of Agricultural and Forestry Sciences, Luoyang 471026, China; 3 Institute of Botany, The Chinese Academy of Sciences, Beijing 100093,China

Received date: 2025-08-11

  Revised date: 2025-11-14

  Online published: 2025-12-08

摘要

牡丹是我国著名特色观赏花卉,花色是决定其观赏和经济价值的重要性状。类黄酮化合物是牡丹花瓣呈色的主要色素。本研究基于杨山牡丹和大花黄牡丹基因组,对参与类黄酮合成的R2R3-MYB转录因子家族中SG4、SG5、SG6、SG7和SG20等亚组成员进行了系统分析,包括进化关系、氨基酸序列特征和内含子插入模式等。结果显示,牡丹类黄酮调控相关R2R3-MYB成员在进化上呈现多元化特征,这可能与其功能分化密切相关。通过矮牵牛花瓣瞬时表达体系,初步明确了不同R2R3-MYB类型在类黄酮合成调控中的功能特性。其中,SG6亚组主要调控以ANS为核心的花青素合成途径;SG5亚组主要参与以ANR为关键节点的原花青素合成;SG7亚组则主要调控以FLS为代表的黄酮醇合成通路;而牡丹SG4亚组成员MYBs-SG4a仍保留正向调控类黄酮合成的能力。此外,研究发现不同成员之间存在竞争或协作关系,协同参与类黄酮合成结构基因的表达调控。本研究为理解植物R2R3-MYB家族的进化与功能多样性提供了新的视角,也为牡丹花色分子育种奠定了理论基础。

关键词: 牡丹; R2R3-MYB; 类黄酮; 花色

本文引用格式

符真珠 , 袁欣 , 高杰 , 王二强 , 王晓晖 , 李艳敏 , 王慧娟 , 王亮生 , 李珊珊 , 张和臣 . 调控类黄酮合成的牡丹R2R3-MYB转录因子鉴定与分析[J]. 植物学报, 0 : 1 -0 . DOI: 10.11983/CBB25146

Abstract

INTRODUCTION: The tree peony (Paeonia × suffruticosa Andrews) is a renowned ornamental plant in China, whose floral color- a key determinant of its economic and aesthetic value- is predominantly governed by flavonoid pigments. 

RATIONALE: To systematically elucidate the molecular basis of flavonoid regulation in tree peonies, this study focused on the R2R3-MYB transcription factor family, specifically members of the SG4, SG5, SG6, SG7, and SG20 subgroups, which are known to be involved in flavonoid biosynthesis. We integrated genomic resources from P. ostii and P. ludlowii to conduct a comprehensive analysis of their evolutionary relationships, amino acid sequence features, and intron insertion patterns. 

RESULTS: The flavonoid-related R2R3-MYB members in tree peonies exhibited remarkable evolutionary diversity, which likely underpins their functional divergence. A transient expression assay in petunia petals revealed distinct regulatory roles for different subgroups. SG6 members primarily regulated the anthocyanin biosynthetic pathway centered on ANS; SG5 members were mainly involved in proanthocyanidin biosynthesis via ANR; and SG7 members predominantly influenced flavonol synthesis by modulating FLS expression. Notably, MYBs-SG4a, one member of the SG4 subgroup, retained the ancestral capacity to positively regulate flavonoid synthesis. Furthermore, we identified both competitive and collaborative interactions among different R2R3-MYB members, which collectively fine-tune the expression of structural genes in the flavonoid pathway. 

CONCLUSION: This study provides novel insights into the evolutionary and functional diversification of the R2R3-MYB family in plants. The findings establish a foundational framework for understanding the complex regulatory network of flavonoid biosynthesis in tree peonies and offer valuable theoretical guidance for molecular breeding aimed at floral color improvement.

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