绣球花筑紫之风离体再生技术体系的建立

金玉妍; 陈双双; 冯景; 刘欣童; 齐香玉; 陈慧杰; 董燕; 邓衍明

doi:10.11983/CBB25068

植物学报 >

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DOI: https://doi.org/10.11983/CBB25068

技术方法

绣球花筑紫之风离体再生技术体系的建立

金玉妍 ,
陈双双 ,
冯景 ,
刘欣童 ,
齐香玉 ,
陈慧杰 ,
董燕 ,
邓衍明

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1江苏省农业科学院休闲农业研究所, 南京 210014; 2中国花卉协会, 北京 100102

收稿日期: 2025-04-17

修回日期: 2025-07-17

网络出版日期: 2025-07-30

基金资助

中央财政林业科技推广示范项目(No.苏[2024]TG03)，江苏省农业科技自主创新资金项目(No.CX(22)2035)

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Establishment of Regeneration System in vitro for Hydrangea macrophylla cv. ‘Chikushi-no-kaze’

JIN Yu-Yan ,
CHEN Shuang-Shuang ,
FENG Jing ,
LIU Xin-Tong ,
ZI Xiang-Yu ,
CHEN Hui-Jie ,
DONG Yan ,
DENG Yan-Meng

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1Institute of Leisure Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 2China Flower Association, Beijing 100102, China

Received date: 2025-04-17

Revised date: 2025-07-17

Online published: 2025-07-30

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摘要

以绣球花筑紫之风(Hydrangea macrophylla ‘Chikushi-no-kaze’)无菌苗叶片为外植体进行高效再生技术研究，探究不同叶位、暗培养时间及植物生长调节剂等条件对愈伤组织诱导、不定芽再生和增殖、壮苗生长及生根的影响。结果表明，筑紫之风组培苗的第3-5位叶(即中部成熟叶片)为最佳取样叶位，暗培养10-14天有利于诱导愈伤组织和再生不定芽，最适培养基为MS+3.0 mg·L-1 CPPU+0.1 mg·L-1 2,4-D，诱导率和再生率分别为97.78%和93.33%；不定芽增殖最适培养基为MS+2.0 mg·L-1 6-BA+0.1 mg·L-1 IBA，增殖系数可达8.33；壮苗生长最适培养基为MS+1.0 mg·L-1 6-BA+0.1 mg·L-1 IBA，幼苗平均茎高为4.10 cm；以MS+0.3 mg·L-1 IBA进行生根培养，生根率可达87.20%。本研究建立了绣球花筑紫之风高效离体再生技术体系，为种苗快繁、品种改良和基因功能研究提供了技术支撑。

关键词： 愈伤组织; 绣球花; 叶片再生; 种苗快繁; 组织培养

本文引用格式

金玉妍 , 陈双双 , 冯景 , 刘欣童 , 齐香玉 , 陈慧杰 , 董燕 , 邓衍明 . 绣球花筑紫之风离体再生技术体系的建立[J]. 植物学报, 0 : 1 -0 . DOI: 10.11983/CBB25068

Abstract

INTRODUCTION: Hydrangea is essential in landscaping, ecology, and medical care, with significant development prospects. ‘Chikushi-no-kaze’ is an ideal low-maintenance variety for micro-landscaping and potted Hydrangea, widely favored by consumers. However, the extremely low setting rate of Hydrangea and poor seed development under natural conditions render traditional reproduction methods inadequate for meeting the demands of large-scale annual production in the market. The breeding of Hydrangea plantlets through tissue culture technology is currently the most efficient method for producing high-quality plantlets.

RATIONALE: Regeneration efficiency in plant tissue culture is a key factor in achieving factory seedling production. Therefore, this study investigated the regeneration efficiency of isolated leaves from tissue culture plantlets of ‘Chikushi-no-kaze’ under optimal culture conditions at each key stage, considering different leaf positions, dark culture durations, and other factors. The aim was to establish an efficient regeneration technology system, which provides technical guidance for large-scale plantlet production and serves as a reference for establishing a genetic transformation system for Hydrangea in the future.

RESULTS: The 3rd to 5th leaves (middle mature leaves) of ‘Chikushi-no-kaze’ tissue culture plantlets were identified as the optimal sampling leaves. A dark culture duration of 10-14 days was conducive to callus formation. The most suitable medium for the induction and regeneration of adventitious buds was MS + 3.0 mg·L-1 CPPU + 0.1 mg·L-12,4-D, with induction and regeneration rates of 97.78% and 93.33%, respectively. The optimal medium for adventitious bud proliferation was MS + 2.0 mg·L-1 6-BA + 0.1 mg·L-1 IBA, yielding a proliferation coefficient of 8.33. For elongation growth, the optimal medium was MS + 1.0 mg·L-1 6-BA + 0.1 mg·L-1 IBA, resulting in an average stem length of 4.10 cm. The optimal medium for rooting culture was MS + 0.3 mg·L-1 IBA, achieving a rooting rate of 87.20%.

CONCLUSION: This study initially established a technical system for in vitro leaf regeneration of big leaf Hydrangea 'Chikushi-no-kaze', which effectively solved the problem of low efficiency of adventitious bud regeneration of Hydrangea, helped to achieve efficient reproduction and recycling. Lay the foundation for large-scale production and genetic improvement of Hydrangea.

Key words： callus; Hydrangea macrophylla ; leaf regeneration; rapid propagation of plantlets; tissue culture

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