黑果腺肋花楸(Aronia melanocarpa)因其观赏价值和经济价值而闻名, 但其系统进化关系仍不明晰。该研究完成了黑果腺肋花楸叶绿体(cp)基因组的测序和分析, 并与13个蔷薇科物种的叶绿体基因组进行了比较分析。黑果腺肋花楸的cp基因组长为159 772 bp, 呈典型的四分结构; 其中大单拷贝区(LSC)长87 810 bp, 小单拷贝区(SSC)为19 200 bp, 中间含有2个26 381 bp的反向重复区(IRa和IRb)。注释到132个基因, 87个蛋白质编码基因、37个tRNA和8个rRNA。还检测到76个简单重复序列(SSR)和50个长重复序列。系统进化分析表明, 黑果腺肋花楸与红果腺肋花楸(A. arbutifolia)的亲缘关系最密切, 与榅桲(Cydonia oblonga)是姊妹支系。该文报道的基因组信息将为进一步研究黑果腺肋花楸的进化、种群遗传分析以及分子育种研究提供新的理论支持。

For the first time, the complete Aronia melanocarpa chloroplast (cp) genome was sequenced and analyzed. A. melanocarpa is well known for its ornamental and economic value; however, the phylogenetic relationships and boundaries between species of Aronia are still uncertain. Chloroplast genomic DNA of A. melanocarpa was extracted and sequenced, analyzed, and compared with that from 13 other species in the Rosaceae family. The A. melanocarpa cp genome is 159 772 bp and exhibits a typical quadripartite structure with four separate regions, including a large single-copy (LSC) region of 87 810 bp and a small single-copy (SSC) region of 19 200 bp separated by two inverted repeats (IRa and IRb) regions of 26 381 bp each. A total of 132 unique genes were annotated, including 87 protein-coding genes, 37 tRNAs, and eight rRNAs. In total, 76 simple sequence repeats (SSRs) and 50 long repeats were detected. Phylogenetic analysis indicated that A. melanocarpa is most closely related to Aronia arbutifolia and forms a sister clade to Cydonia oblonga with weak support. The genome information reported here should provide a new resource for further research into the evolution and population genetic analyses of A. melanocarpa, as well as molecular breeding studies.