[an error occurred while processing this directive] [an error occurred while processing this directive] [an error occurred while processing this directive]
[an error occurred while processing this directive]

植物学报 >

0 1 - 0

DOI: https://doi.org/10.11983/CBB24113

技术方法

西印度醋栗组培快繁技术初探

  • 李彤 ,
  • 李楚然 ,
  • 张芷瑜 ,
  • 付晓熳 ,
  • 刘云 ,
  • 杨晓琴 ,
  • 张颖君 ,
  • 杨力颖 ,
  • 赵平
展开

  • 1西南林业大学西南地区林业生物质资源高效利用国家林业和草原局重点实验室, 昆明 650224; 2中国科学院昆明植物研究所植物化学与天然药物重点实验室, 昆明 650201; 3云南新兴绿化工程有限公司, 昆明 650228


收稿日期: 2024-07-23

  修回日期: 2024-10-29

  网络出版日期: 2024-12-27

基金资助

国家自然科学基金(No.32270426; No.82074124)

收起

A Preliminary Study on Tissue Culture and Rapid Propagation Technology of Phyllanthus acidus

  • LI Tong ,
  • LI Chu-Ran ,
  • ZHANG Zhi-Yu ,
  • FU Xiao-Man ,
  • LIU Yun ,
  • YANG Xiao-Qin ,
  • ZHANG Ying-Jun ,
  • YANG Li-Ying ,
  • DIAO Beng
Expand

  • 1Key Laboratory of State Forestry and Grassland Administration on Highly-Efficient Utilization of Forestry Biomass Resources in Southwest China, Southwest Forestry University, Kunming 650224, China; 2Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; 3Collaborative Yunnan Xinxing Greening Engineering Co., Ltd., Kunming 650228, China

Received date: 2024-07-23

  Revised date: 2024-10-29

  Online published: 2024-12-27

Fold

摘要

西印度醋栗(Phyllanthus acidus (L.) Skeels)是一种生长于热带地区的乔木, 兼具食用和药用价值, 开展其组培快繁技术探究, 可促进其资源的持续开发和利用。本研究以西印度醋栗茎尖为外植体材料, 对初代培养、继代增殖培养及生根培养的方案进行了初步筛选。结果表明, 初代培养最适培养基为MS+2.0 mg·L–1 6-BA+0.2 mg·L–1 NAA, 外植体诱导萌发率达81.11%; 继代增殖最适培养基为MS+1.0 mg·L–1 6-BA+0.2 mg·L–1 IBA, 增殖系数达1.86; 生根最适培养基为MS+1.5 mg·L–1 IBA, 生根率达83.00%; 炼苗7天后移栽, 移栽基质为珍珠岩、泥炭土、腐殖质=1:1:1 (v/v/v), 成活率为90%。从接种外植体到获得再生植株约90天时间, 为西印度醋栗的资源保护与产业化应用提供了技术支持。

关键词： 西印度醋栗; 组织培养; 快速繁殖

本文引用格式

李彤 , 李楚然 , 张芷瑜 , 付晓熳 , 刘云 , 杨晓琴 , 张颖君 , 杨力颖 , 赵平 . 西印度醋栗组培快繁技术初探[J]. 植物学报, 0 : 1 -0 . DOI: 10.11983/CBB24113

Abstract

Phyllanthus acidus (L.) Skeels is a tropical tree with both edible and medicinal properties. Exploring its tissue culture and rapid propagation technology can promote the sustainable development and utilization of its resources. In this study, the stem tips of P. acidus were used as explants, and the schemes of primary culture, secondary proliferation culture and rooting culture were screened. The results showed that the optimal medium for primary culture was MS+2.0 mg·L–1 6-BA+0.2 mg·L–1 NAA, and the induced germination rate of explants reached 81.11%. The optimal medium for subculture was MS+1.0 mg·L–1 6-BA+0.2 mg·L–1 IBA, and the proliferation coefficient was 1.86. The optimal medium for rooting was MS+1.5 mg·L–1 IBA, and the rooting rate reached 83.00%. After 7 days of cultivation, the seedlings were transplanted with perlite, peat soil and humus=1:1:1 (v/v/v), and the survival rate was 90%. This study took about 90 days from inoculation of explants to obtaining regenerated plants, providing technical support for the resource conservation and industrial application of P. acious.

Key words： Phyllanthus acidus; tissue culture; rapid propagation

[an error occurred while processing this directive]

参考文献

[1]陈翠红, 苗春泽, 田年军, 刘学芝, 张永红, 陈翠楠, 尚军烨(2023).植物组织培养中常见污染类型及污染防控措施.现代园艺, 46:155-156. [2]陈剑英, 邓桂香(2024).西印度醋栗播种育苗关键技术.林业科技通讯, 67:119-120. [3]顾晨 (2019).西双版纳产西印度醋栗化学成分研究. 硕士论文. 昆明: 昆明理工大学. pp. 1–89.., :-. [4]黄雯, 林亮通, 许传俊, 郑志忠, 陈良华, 明艳林(2017).叶下珠组培快繁体系研究.亚热带植物科学, 46:236-239. [5]黄欣艳, 蒙小玉, 冯建文, 乔光, 吴亚维(2024).平邑甜茶离体快繁体系的建立., 53:-. [6]黄勇, 张铁, 沈尚东 (2018).正交试验优化余甘子愈伤组织诱导培养基研究.甘肃农业科技, 56:49-51. [7]侯润庚, 丁骁, 杨力颖, 张曼, 张颖君, 赵平(2023).西印度醋栗枝叶提取物的α-葡萄糖苷酶抑制活性及安全性评价.食品工业科技, 44:252-259. [8]李佳慧, 叶维雁, 朱鹏锦, 庞新华, 张继, 唐毓玮, 韦俏宇(2022).猫须草无菌短枝组织培养与快速繁殖体系的建立.热带作物学报, 33:2063-2070. [9]缪煜灿, 王海燕(2021).叶下珠属植物抗肿瘤作用的研究进展.中国中医药现代远程教育, 19:189-192. [10]凌雪, 张迪, 严雪龙, 濮社班(2015).西印度醋栗化学成分及活性研究进展.中国野生植物资源, 34:40-43. [11]刘东奇(2013).植物组织培养过程中生长素和细胞分裂素的配比问题浅析.中学生物学, 29:5-6. [12]罗丹丹, 顾婷, 李西文, 段宝忠(2017).傣医学药用大戟科植物药材品种与标准的现状分析.中药新药与临床药理, 18:692-698. [13]普天磊, 金杰, 马开华, 袁建民, 罗会英, 赵琼玲(2023).西印度醋栗表型性状及营养成分测定分析.热带农业科学, 34:47-51. [14]唐链, 田爽琪(2022).植物组织培养技术的应用进展.现代园艺, 45:24-26. [15]王政, 张春玲, 何松林, 尚文倩, 贺丹, 申玉晓, 孙宇科(2022).不同反光及照光方式对非洲菊组培苗生长的影响.河南农业科学, 51:120-129. [16]文书生, 成仿云, 钟原, 王新, 李刘泽木, 黄弄璋(2016).正午’牡丹微繁殖体系的建立.植物科学学报, 34:143-150. [17]胥佳, 李娜, 朱宏涛, 杨慰农, 王东, 杨崇仁, 张颖君(2021).西印度醋栗的化学成分及药理活性研究进展.广西植物, 47:1784-1793. [18]张宝华(2023).植物组织培养技术及应用探究.现代农业研究, 29:90-93. [19]张守英, 姚小华, 任华东, 王开良(2002).余甘子离体快速繁殖技术的初步研究.林业科学研究, 15:116-119. [20]张伟彬(2022).消毒剂种类及消毒时间对牡丹外植体的影响.农技服务, 39:46-48. [21]张小斌, 雷艳妮, 陈书存 (2018).不同生态条件对叶下珠种子萌发的影响研究.陕西农业科学, 64:40-42. [22]赵永华, 丁赢, 杨春清, 郭玉海, 解超杰(2000).我国叶下珠属药用植物资源的开发利用.生物学通报, 49:39-40. [23]Andrianto D, Widianti W, Bintang M(2017).Antioxidant and cytotoxic activity of Phyllanthus acidus fruit extracts.IOP Conference Series: Earth and Environmental Science, 58:012022-. [24]Chakraborty R, De B, Devanna N, Sen S(2012).Antiinflammatory, antinociceptive and antioxidant activities of Phyllanthus acidus L. extracts.Asian Pacific Journal of Tropical Biomedicine, 2:S953-S961. [25]Duong H, Nguyen H, Nguyen T, Bui H(2019).Triterpenoids from Phyllanthus acidus (L.) Skeels.Science and Technology Development Journal: Natural Sciences, 2:71-75. [26]Geng HC, Zhu HT, Yang WN, Wang D, Yang CR, Zhang YJ(2021).New cytotoxic dichapetalins in the leaves of Phyllanthus acidus: Identification, quantitative analysis, and preliminary toxicity assessment.Bioorganic Chemistry, 114:105125-. [27]Leeya Y, Mulvany MJ, Queiroz EF, Marston A, Hostettmann K, Jansakul C(2010).Hypotensive activity of an n-butanol extract and their purified compounds from leaves of Phyllanthus acidus (L.) Skeels in rats.European Journal of Pharmacology, 649:301-313. [28]Longuefosse JL, Nossin E(1996).Medical ethnobotany survey in Martinique.Journal of Ethnopharmacology, 53:117-142. [29]Murashige T, Skoog F(1962).A revised medium for rapid growth and bio assays with tobacco tissue cultures.Physiologia Plantarum, 15:473-497. [30]Panda J, Mishra AE(2021).A short review on pharmacological properties of Phyllanthus acidus (L.) Skeels.International Journal of Botany Studies, 6:403-405. [31]Shimu AS, Miah M, Billah M, Karmakar S, Mohanto SC, Khatun R, Reza MA, Hoque KMF(2021).A comparative study of biological potentiality and EAC cell growth inhibition activity of Phyllanthus acidus (L.) fruit pulp and seed in Bangladesh.Saudi Journal of Biological Sciences, 28:2014-2022. [32]Tan SP, Tan ENY, Lim QY, Nafiah MA(2020).Phyllanthus acidus (L.) Skeels: A review of its traditional uses, phytochemistry, and pharmacological properties.Journal of Ethnopharmacology, 253:112610-. [33]Xu J, Xin Y, Zhu HT, Kong QH, Yang WN, Wang D, Yang CR, Zhang YJ(2023).Flavonoids from the fruits of Phyllanthus acidus (L.) Skeels with anti-α-glucosidase activity.Natural Product Research, 37:1986-1992. [34]Zhu GY, Luo D, Zhao YQ, Xiang ZR, Chen C, Li N, Hao XJ, Ding X, Zhang YJ, Zhao YH(2024).Pacidusin B isolated from Phyllanthus acidus triggers ferroptotic cell death in HT1080 cells.Natural Products and Bioprospecting, 14:34-.
Options
文章导航

/

〈 〉

[an error occurred while processing this directive]