植物学报 ›› 2000, Vol. 17 ›› Issue (03): 260-265.

• 研究简报 • 上一篇    下一篇

十字花科10属种与油菜萝卜胞质不育系杂交的花粉萌发情况观察

魏琴 周黎军 陈东林 李旭峰 陈放   

  1. (宜宾师范高等专科学校 宜宾 644007)(四川大学生命科学学院 成都 610064)(四川大学生命科学学院 成都 610064)
  • 收稿日期:1999-08-17 修回日期:2000-01-23 出版日期:2000-05-20 发布日期:2000-05-20
  • 通讯作者: 周黎军

The Observation on Pollen Germination in Crossings Between Ten Taxa of Cruciferae and Ogu CMS

WEI Qin ZHOU Li-JunCHEN Dong-Lin LI Xu-Feng CHEN Fang   

  1. (Department of Biology, Yibin Terchers College,Yibin 644007) (College of Life Science,Sichuan University, Chengdu 610064)
  • Received:1999-08-17 Revised:2000-01-23 Online:2000-05-20 Published:2000-05-20
  • Contact: ZHOU Li-Jun

摘要: 对10属种十字花科植物与油菜萝卜胞质不育系杂交时花粉在柱头上粘合、萌发、花粉管伸长等情况进行观察。结果表明:(1)海甘蓝花粉粒粘合较难;(2)48 h内无瓣焊菜〖WTBX〗(Rorippa dubia)〖WTBX〗、毛果诸葛菜(Orychophragmus violaceus)、桂竹香(Cheiranthus cheiri)、海甘蓝(Crambe abyssinica)花粉管的伸长受阻于花粉萌发启动之时,花粉壁内形成胼胝质塞;播娘蒿、紫罗兰、荠菜花粉管伸长但未进入乳突细胞;芝麻菜花粉管进入乳突细胞而未进入柱头,‘浠水白’(Brassica campestris)、蓝花子有花粉管进入柱头及花柱而未进入胚囊。

Abstract: Pollen grain adhesion, germination and pollen tube growth were observed in intergeneric crosses between ten taxa and Ogu CMS.The pol len grains of Crambe abyssinica hardly adhered to the stigma of Og u CMS. Observations made within 48 h showed that the pollen tubes of Rorippa dubia(Pers.) Hara, Orychophragmus violaceus var. lasiocarpus Migo, Cheriranthus cheiri L.and Crambe abyssisca were obstructed and callose plugs formed in sporoderm at the start of pollen germination. The pollen tubes of Descurainia sophia(L.) Webb. ex Prantl, Matthiola incana(L.) R. Br.and Capsella bursa_pastoris(L.) Medic grew but failed to penetrate the papilla cells. The pollen tubes of Eruca sativa Mill. var. sativa penetrated the papilla cells, but did not penetrate the stigma. The pollen tubes of Brassica campestris huibai’ and Raphanus sativus L.var raphanistroides(Makino) Makino entered the stigmata and styles but did not penetrate the embryo sacs.