Abstract: Four hybrids of Vitis amurensis cv. ‘Zuoshan1’בSO4’ (A11, A14, A15, and A17) were used as materials (rootstock 1103 of Paulsen and Eurasian cultivars V. vinifera cv. ‘Crimson’ were used as double controls. Crimson alkali resistance is stronger than that of rootstocks, and 1103P is stronger in rootstocks) to be irrigated with 100 mmol·L^–1 NaHCO₃. Chlorophyll fluorescence and P700-induced curve treatment were determined to analyze the photosystem activity response of these hybrids under alkaline salt treatment. The F_v/F_m, qP, F_v'/F_m' and P_m of Crimson, A14 and A15 decreased less under NaHCO₃ stress, the Y(NO) of Crimson, A14 and A15 increased less, and the fluorescence parameters had larger change in A11, A17 and 1103P. The ETR(I)–ETR(II) value was maintained at a higher level in A14 and A15 under NaHCO₃ stress and alleviated the inhibition of the alkaline system on the activity of the light system. Principal component analysis of the fluorescence parameters indicated that A15 had strong alkali resistance, A14 and Crimson had medium alkali resistance, and the alkali resistance of A11, A17 and 1103P was weaker, which was consistent with the classification by biomass.