苦皮藤试管苗生根培养研究

植物学报 ›› 2004, Vol. 21 ›› Issue (03): 332-336.

苦皮藤试管苗生根培养研究

马艳肖娅萍王彩玲王哲之

1（陕西师范大学生命科学学院西安 710062） 2（武汉钢铁公司子女学校武汉 430080）

收稿日期:2003-07-30 修回日期:2004-01-12 出版日期:2004-06-20 发布日期:2004-06-20
通讯作者: 肖娅萍

The Study of Root Induction in Celastrus angulatus in vitro

MA Yan XIAO Ya-Ping WANG Cai-Ling WANG Zhe-Zhi

1(College of Life Science, Shaanxi Normal University, Xi’an 710062) 2(The appendant shool of Wugang group, Wuhan 430080)

Received:2003-07-30 Revised:2004-01-12 Online:2004-06-20 Published:2004-06-20
Contact: XIAO Ya-Ping

摘要/Abstract

摘要： 探讨了培养因子对诱导苦皮藤(Celastrus angulatus Maxim)试管苗生根的作用，采用正交试验设计法测试了苦皮藤生根关键因素多效唑(MET)、吲哚丁酸(IBA)、暗培养及培养基中盐浓度（简称培养基）的效应。方差分析结果显示，暗培养对苦皮藤生根作用极显著，因子作用大小依次为：暗培养＞培养基＞MET×IBA＞MET＞IBA×培养基＞IBA。诱导苦皮藤组培苗生根的最佳因素配比为：1/2 MS+MET 3.0 mg.L-1 + IBA 0.8 mg.L-1和1/2 MS +MET 3.0 mg.L-1 + IBA0.5 mg.L-1，暗培养12 d效果最好。

Abstract: In this work, the effects of culture factors ( MET, IBA, culture medium and culturing in dark) on root formation were studied in tissue culture of Celastrus angulatus. The results showed that the root induction was significantly affected by duration of culture in dark, while other factors were least effective. The order of their efficiencies was: duration of culture in dark＞culture medium ＞MET×IBA＞MET＞IBA.The best culture medium for root induction of Celastrus angulatus was that containing: 1/2 MS+MET3.0 mg.L-1+IBA 0.8 mg.L-1 or 1/2 MS+ MET3.0 mg.L-1 + IBA 0.5mg.L-1 for 12 days, in dark.

马艳肖娅萍王彩玲王哲之. 苦皮藤试管苗生根培养研究. 植物学报, 2004, 21(03): 332-336.

MA Yan XIAO Ya-Ping WANG Cai-Ling WANG Zhe-Zhi. The Study of Root Induction in Celastrus angulatus in vitro. Chinese Bulletin of Botany, 2004, 21(03): 332-336.

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