不同水氮梯度下典型挺水植物叶绿素荧光的响应特性

doi:10.11983/CBB15096

植物学报 ›› 2016, Vol. 51 ›› Issue (5): 631-638.DOI: 10.11983/CBB15096

不同水氮梯度下典型挺水植物叶绿素荧光的响应特性

宫兆宁^1,^2,^3,^4,^*(), 范云豹^1,^2,^3,⁴, 刘辉^1,^2,^3,⁴, 赵文吉^1,^2,^3,⁴

¹首都师范大学资源环境与旅游学院, 北京 100048
²三维信息获取与应用教育部重点实验室, 北京 100048
³资源环境与地理信息系统北京市重点实验室, 北京 100048
⁴北京市城市环境过程与数字模拟国家重点实验室培育基地, 北京 100048

收稿日期:2015-06-01 接受日期:2016-04-26 出版日期:2016-09-01 发布日期:2016-09-27
通讯作者: 宫兆宁
作者简介:
# 共同第一作者
基金资助:
国家自然科学基金青年科学基金(No.41101404)和国家国际科技合作专项(No.2014DFA21620)

Chlorophyll Fluorescence Response Characteristics of Typical Emergent Plants Under Different Total Nitrogen Gradient

Zhaoning Gong^1,2,3,4*, Yunbao Fan^1,2,3,4, Hui Liu^1,2,3,4, Wenji Zhao^1,2,3,4

¹College of Resource Environment and Tourism, Capital Normal University, Beijing 100048, China
²Key Laboratory of 3D Information Acquisition and Application of Ministry of Education, Beijing 100048, China
³Key Laboratory of Resources Environment and GIS of Beijing Municipal, Beijing 100048, China
⁴Base of the State Laboratory of Urban Environmental Processes and Digital Modeling, Beijing 100048, China

Received:2015-06-01 Accepted:2016-04-26 Online:2016-09-01 Published:2016-09-27
Contact: Gong Zhaoning
About author:
# Co-first authors

摘要/Abstract

摘要： 叶绿素荧光测量分析可以揭示植物叶片光化学效率的变化, 已越来越多地应用于植物生态监测。以再生水为主要补给水源的北京门城湖湿地公园为研究区, 选取典型湿地挺水植物芦苇(Phragmites australis)、香蒲(Typha angustifolia)和茭白(Zizania latifolia)为研究对象, 通过野外测量叶片尺度的叶绿素荧光参数和室内测定对应样点的水体总氮含量指标, 研究了再生水补给条件下, 不同水氮梯度植物叶绿素荧光的响应特性。结果表明, 3种典型挺水植物的初始荧光(F_o)与最大荧光(F_m)随着水体总氮含量的增加呈现上升的趋势; PSII的量子效率(F_v/F_m)与实际量子效率(ΦPSII)受水氮含量的影响先升高, 达到15-20 mg·L^-1区间时, 则与之持平; 光化学淬灭(qP)参数则呈现先升高后降低的变化趋势, 而非光化学淬灭(NPQ)参数的变化没有明显的规律。当水氮含量为15-20 mg·L^-1时, 光化学反应减弱, 光合作用出现抑制。不同类型植物的荧光参数也有所不同, 处于生长期(6月)植物的光合作用显著强于生长成熟期(9月)。

Abstract: Measurements and analysis of chlorophyll fluorescence can reflect changes in plant leaf photochemical efficiency and have been increasingly used in plant ecological monitoring. This study used the Mencheng Lake wetland park, supplied with reclaimed water, as a study area, and Phragmites australis, Typha angustifolia and Zizania latifolia as typical emergent plants. We studied the chlorophyll fluorescence response characteristics of plants under different total nitrogen gradient by measuring leaf-scale chlorophyll fluorescence parameters outdoors and the corresponding water total nitrogen content indoors. Minimal fluorescence (F_o) and maximal fluorescence (F_m) were increased and nitrogen content in water was elevated. Maximum quantum efficiency of photochemistry in photosystem II in the dark (F_v/F_m) and quantum efficiency of PSll (ΦPSll) affected by nitrogen increased at first and then plateaued when nitrogen content reached the 15 to 20 mg·L^-1 interval. Photochemical quenching (qP) tended to decrease after the first increase, but nonphotochemical quenching (NPQ) changed without obvious regularity. When water total nitrogen content was at the 15-20 mg·L^-1 level, photochemical reaction was abated and photosynthesis was inhibited. Also, fluorescence parameters of different types of emergent plants were not the same; photosynthesis was significantly stronger in the growth stage (June) than the mature stage (September).

宫兆宁, 范云豹, 刘辉, 赵文吉. 不同水氮梯度下典型挺水植物叶绿素荧光的响应特性. 植物学报, 2016, 51(5): 631-638.

Zhaoning Gong, Yunbao Fan, Hui Liu, Wenji Zhao. Chlorophyll Fluorescence Response Characteristics of Typical Emergent Plants Under Different Total Nitrogen Gradient. Chinese Bulletin of Botany, 2016, 51(5): 631-638.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB15096

https://www.chinbullbotany.com/CN/Y2016/V51/I5/631

图/表 5

图1 研究区地理位置及采样点的空间分布

Figure 1 Location map and sampling sites of study area

表1 门城湖湿地公园水质状况

Table 1 Water quality status in Mencheng Lake wetland park

Water quality parameters	Minimal value	Maximal value	Mean value	Standard deviation	Class II	Class V
pH	8.78	9.90	9.34	0.32	6-9	6-9
Dissolved oxygen (mg·L^-1)	2.53	10.53	6.23	2.03	6	2
Total nitrogen (mg·L^-1)	5.73	20.10	16.50	5.11	0.50	2.00

图2 水体总氮含量对3种植物Fo和Fm的影响 Fo: 初始荧光; Fm: 最大荧光

Figure 2 Responses of Fo and Fm in leaf of three plants to total nitrogen content in water Fo: Minimal fluorescence yield of the dark-adapted state; Fm: Maximal fluorescence yield of the dark-adapted state

表2 水体总氮含量对3种植物Fv/Fm和ΦPSII的影响

Table 2 Responses of Fv/Fm and ΦPSII in leaf of three plants to total nitrogen content in water

Vegetation type	Water total nitrogen content (mg·L^-1)	Number of samples	June		September
Vegetation type	Water total nitrogen content (mg·L^-1)	Number of samples	F_v/F_m	ΦPSII	F_v/F_m	ΦPSII
Phragmites australis	5-10	20	0.49	0.31	0.46	0.27
	10-15	24	0.60	0.40	0.55	0.37
	15-20	36	0.58	0.43	0.57	0.41
Typha angustifolia	5-10	12	0.59	0.35	0.55	0.30
	10-15	8	0.70	0.47	0.60	0.39
	15-20	20	0.71	0.49	0.59	0.38
Zizania latifolia	5-10	6	0.48	0.36	0.34	0.34
	10-15	12	0.59	0.33	0.54	0.36
	15-20	14	0.60	0.25	0.56	0.32

图3 水体总氮含量对3种植物qP和NPQ的影响 qP: 光化学淬灭; NPQ: 非光化学淬灭

Figure 3 Responses of qP and NPQ in leaf of three plants to total nitrogen content in water qP: Photochemical quenching; NPQ: Nonphotochemical quenching

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不同水氮梯度下典型挺水植物叶绿素荧光的响应特性

Chlorophyll Fluorescence Response Characteristics of Typical Emergent Plants Under Different Total Nitrogen Gradient

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