植物学报 ›› 2001, Vol. 18 ›› Issue (01): 52-57.

• 综述与专论 • 上一篇    下一篇

mRNA差别显示技术在植物发育研究中的应用

闫桂平 马凤鸣 韩天富 李文华   

  1. 1(东北农业大学农学系, 哈尔滨 150030)2(中国农业科学院作物育种栽培研究所, 北京 100081)
  • 收稿日期:1999-10-28 修回日期:1999-12-29 出版日期:2001-01-20 发布日期:2001-01-20
  • 通讯作者: 闫桂平

Applications of mRNA Differential Display in the Studies on Plant Development

YAN Gui-PingMA Feng-MingHAN Tian-FuLI Wen-Hua   

  1. 1(Department of Agronomy, Northeast Agricultural University, Harbin 150030)2(Institute of Crop Breeding and Cultivation, Chinese Academy of Agricultural Sciences, Beijing 100081)
  • Received:1999-10-28 Revised:1999-12-29 Online:2001-01-20 Published:2001-01-20
  • Contact: YAN Gui-Ping

摘要: mRNA差别显示技术是近年发展起来的研究基因表达差异的有效方法。它不仅可比较基因表达差异、追踪已知基因的表达情况,而且可作为寻找特异表达基因的重要手段。本文介绍此方法在植物春化作用、光周期反应、花发育、雄性不育、成熟、衰老、种子发育和休眠及根发育等研究领域的应用概况。

Abstract: mRNA differential display technique is an effective method to analyze differential gene expression and to seach the genes which expressed specially. This paper briefly reviews the recent progress of applications of this method in research on plant developmental, such as the molecular mechanism of vernalization, photoperiod response, the development of root, flower and seed, male sterility, senescence, fruit maturation, etc.