植物学报 ›› 2014, Vol. 49 ›› Issue (5): 587-594.DOI: 10.3724/SP.J.1259.2014.00587

• 技术方法 • 上一篇    下一篇

农杆菌介导的棉花子叶瞬时表达系统的建立

刘雪梅, 王蕾, 文添龙, 何鹏, 俞嘉宁*   

  1. 陕西师范大学生命科学学院, 西安 710119
  • 收稿日期:2013-08-01 修回日期:2013-11-16 出版日期:2014-09-01 发布日期:2014-07-02
  • 通讯作者: 俞嘉宁
  • 基金资助:

    国家自然科学基金;国家重点基础研究发展计划;陕西省农业公关

The Establishment of Agrobacterium-mediated Transient Expression System in Cotton Cotyledons

Xuemei Liu, Lei Wang, Tianlong Wen, Peng He, Jianing Yu*   

  1. College of Life Sciences, Shaanxi Normal University, Xi’an 710119, China
  • Received:2013-08-01 Revised:2013-11-16 Online:2014-09-01 Published:2014-07-02
  • Contact: Jianing Yu

摘要: 植物瞬时表达系统在研究功能基因的亚细胞定位、蛋白质互作、启动子活性等方面具有重要作用。选取棉花(Gossypium hirsutum)子叶为材料, 以β-葡糖醛酸酶基因(GUS)、增强型绿色荧光蛋白基因(eGFP)为报告基因, 对棉花子叶生长时间、农杆菌浓度、注射量、共培养时间等因素进行了分析, 建立了农杆菌介导的棉花子叶瞬时表达系统。结果表明, 对萌发6天的棉花子叶注射50 μLOD600为0.5的含目的基因的农杆菌LBA4404或GV3101, 共培养2–3天后, 外源基因表达效率最高。利用此方法从棉花萌发到观测结果, 仅需8–13天, 且操作简单易行, 可快速检测棉花来源的启动子活性、亚细胞定位、蛋白质互作等。该方法在棉花基因功能研究方面有很好的应用价值。

Abstract: The plant transient expression system plays an important role in analysis of subcellular localization, interaction and promoter activity of protein. Here, we established a transient expression system in cotton cotyledons based on Agrobacterium-mediated transformation with GUS and eGFP as reporter genes. We investigated the optimal conditions for the system, including cotyledon growth time, Agrobacterium concentration, injection volume and co-culture time. The highest efficiency of exogenous gene expression was 50 μL Agrobacterium LBA4404 or GV3101 with OD600=0.5 and co-culture for 2–3 days in cotton cotyledons germinated for 6 days. The transient expression system is convenient and only needs 8 to 13 days to detect exogenous protein expression from germination to results. The system may have a good application for analysis of gene function in cotton.