植物学报 ›› 1990, Vol. 7 ›› Issue (04): 27-30.

• 研究报告 • 上一篇    下一篇

发菜藻胆体分离和光谱特性的研究

路荣昭 刘斌 张正东   

  1. 1(中国科学院植物研究所,北京 100044) 2(中国科学院研究生院,北京)
  • 出版日期:1990-12-10 发布日期:1990-12-10

Studies on the Isolation and Spectral Characteristics of Phycobllisomes From Nostoc flagelliforme

Lu Rong-zhao, Liu Bin and Zhang Zheng-dong   

  1. 1(Institute of Botany, Academia Sinica, Beijing 100044) 2(Graduate School, Academia Sinica, Beijing)
  • Online:1990-12-10 Published:1990-12-10

摘要: 完整藻胆体和不完整藻胆体的吸收峰都在618nm。完整藻胆体的室温荧光峰位于670nm 以上,而不完整藻胆体则在670nm以下。完整藻胆体的77K荧光发射光谱中只有648nm一个荧光发射带;而在不完整藻胆体,则有2个或3个发射带,它们位于684nm,666nm和648nm, 依次属于别藻蓝蛋白 — B,别藻蓝蛋白和C — 藻蓝蛋白的荧光。

Abstract: Absorption maxima of intact and non-intact phycobilisomes are located at 618nm. The room temperature fluorescence emission maximum of intact phycobilisomes is located at more .than 670nm; that of non-intact phycobilisomes is less than 670nm. There is only one 684 nm band in 77 K fluorescence emission spectrum of intact phycobilisomes; two or three bands in that of non-intact phycobilisomes, they are located at 684 nm, 666 nm and 648 nm,characteristics of allophycocyanin-B, allophycocyanin and C-phy-cyanin respectively.