Abstract: The effect of boron deficiency on ethylene release of cotton, cucumber and rape were studied. The results showed: Under boron deficiency condition, ethylene release of cotton and cucumber was increased, but ethylene release of rape was not affected, the increase of ethylene release inhibited the growth of cotton and cucumber, spraying CoCl2 and STS can relieve the effect of boron deficiency on cotton and cucumber; ethylene release of cucumber increased after cucumber had boron deficiency symptoms, so increase of ethylene release is not the direct reason of resulted cucumber disorder.