植物学报 ›› 1998, Vol. 15 ›› Issue (02): 50-54.

• 研究报告 • 上一篇    下一篇

抗寒剂CR-4 对冬小麦幼苗质膜钙泵(Ca2 +-ATPase) 的稳定作用

孙德兰 王红 简令成   

  1. (中国科学院植物研究所 北京 100093)
  • 出版日期:1998-03-20 发布日期:1998-03-20
  • 通讯作者: 孙德兰

The Stabilizaton on the Plasmalemma Calcium-Pump( Ca2 + -ATPase) in Winter Wheat Seedlings by the Cold-Resistant Agent CR-4

SUN De-Lan, WANG Hong and JIAN Ling-Cheng   

  1. (Institute of Botany, The Chinese Academy of Science, Beijing 100093)
  • Online:1998-03-20 Published:1998-03-20
  • Contact: SUN De-Lan

摘要: 通过磷酸铈沉淀的细胞化学观察揭示,常温下生长的冬小麦幼苗的Ca2+ -ATP酶活性主要定位在质膜上,同时,水浸种和抗寒剂浸种的小麦质膜Ca2+ -ATP酶活性没有差异。然而,小麦幼苗经-7℃冰冻处理12小时和24小时后,则表现明显的区别:水浸种的小麦幼苗质膜Ca2+ -ATP酶活性明显下降,直至完全失活,细胞的精细结构也同时被破坏;而经抗寒剂浸种的小麦幼苗质膜Ca2+ -ATP酶仍维持较高的活性,细胞结构也保持完整,显示抗寒剂对质膜Ca2+ -ATPase酶起着明显的稳定作用。

Abstract: The electron microscopical observations using Cytochemical method of cerium phosphate precipitation indicated that the Ca2+-ATPase activity was mainly localized at the plasmalemma in wheat seedling cells grown at normal temperature. And the enzyme activity did not appear to be different between water-soaked and CR4-Soaked treatments under normal temperature. However, the differences occurred when wheat seedlings were treated for 12 hrs and 24 hrs at - 7℃: the plasmalemma Ca2+-ATPase activity in the seedlings from water-soaked seeds was markedly decreased, and the ultrastructures were disrupted, and that from cold-resistant agent CR4-Soakod seeds was still maintained, and their free structures were also intact. The results suggest that the cold-resistant agent CR-4 plays an important role for stabilizing plasmalemma calcium pump (Ca2+-ATPase) under low temperature stress.