植物学报 ›› 2002, Vol. 19 ›› Issue (06): 705-709.

• 研究论文 • 上一篇    下一篇

生长素结合蛋白cDNA的克隆及其在黄瓜中的表达

白吉刚 宋明 刘佩瑛 张盛林 王中凤   

  1. (西南农业大学园艺系 重庆 400716)
  • 收稿日期:2001-12-21 修回日期:2002-02-04 出版日期:2002-11-20 发布日期:2002-11-20
  • 通讯作者: 刘佩瑛

Cloning of Auxin_binding Protein 1 cDNA and Its Expression in Cucumber

BAI Ji-Gang SONG Ming LIU Pei-YingZHANG Sheng-Lin WANG Zhong-Feng   

  1. (Horticultural Department, Southwest Agricultural University, Chongqing 400716)
  • Received:2001-12-21 Revised:2002-02-04 Online:2002-11-20 Published:2002-11-20
  • Contact: LIU Pei-Ying

摘要: 利用RT_PCR方法从拟南芥(Arabidopsis thaliana (L.) Heynh.)中克隆了生长素结合蛋白(auxin binding protein 1)cDNA,进行了全序列测定。将该基因克隆在pBI121的35 S启动子和Nos终止子之间,得到植物表达载体p35EZ。通过根癌农杆菌(Agrobacterium tumefaciens (Smith et Townsend) Conn)介导的方法转化黄瓜(Cucumis sativus L.)。对转基因植株进行了PCR和Southern检测。所得到的转基因黄瓜植株单性结实能力增强。

Abstract: An auxin-binding protein 1 cDNA was isolated from Arabidopsis thaliana (L.) Heynh.by RT_PCR and confirmed by DNA sequencing. Plant expression vector p35EZ was constructed to transform the explants of Cucumis sativus L. by Agrobacterium tumefaciens (Smith et Townsend) Conn Ti plasmid_mediated method. The intergration of the transgene was identified by PCR and Southern blot analysis. It was found that the parthenocarpic ability of transgenic cucumber plants was enhanced.