植物学报 ›› 2002, Vol. 19 ›› Issue (02): 194-200.

• 研究论文 • 上一篇    下一篇

桑树二倍体及人工诱导的同源四倍体遗传差异的AFLP分析

王卓伟 余茂德 鲁成   

  1. (农业部蚕桑学重点实验室,西南农业大学 重庆 400716)
  • 收稿日期:2001-03-26 修回日期:2001-08-15 出版日期:2002-03-20 发布日期:2002-03-20
  • 通讯作者: 余茂德

The AFLP Analysis of Genetic Diversity between Diploid and Artificially Induced Homologous

WANG Zhuo-Wei YU Mao-De LU Cheng   

  1. (The key Sericultural Laboratory of Agriculture Ministry, Southwest Agricultural University, Chongqing 400716)
  • Received:2001-03-26 Revised:2001-08-15 Online:2002-03-20 Published:2002-03-20
  • Contact: YU Mao-De

摘要: 利用AFLP(Amplified Fragment Length Polymorphism)分子标记技术,即扩增片段长度多态性,从DNA分子水平探讨二倍体桑(2n=2X=28)与经秋水仙素诱变得到的同源四倍体桑(2n=4X=56)在遗传结构上的差异。根据对供试材料DNA多态性及遗传距离分析,认为经秋水仙素诱变得到的同源4X与2X相比在DNA分子遗传结构上产生一定程度的改变,在种内变异水平上,2X与同源4X桑间的遗传差异小于种间差异。

Abstract: AFLP molecular markers is used to analyze the genetic diversity betwee n diploid and homologous tetraploid of mulberry chemically induced with by colchicines. According to the analysis of DNA polymorphism and genetic distance of assayed materials , the genetic diversity between diploid and homologous tetraploid is less than that of interspecies and is in the range of intraspecies diversity. It can be concluded that the DNA molecular genetic structure is changed by colchicines.