Abstract: A data acquisition and data analysis software for millisecond chlorophyll fluorescence kineics of plant is introduced, based on an IBM PC microcomputer. This software is written in the C language with Chinese interface. It can be used very easily in the menu-driven mode with color windows and has fine flexibility and compatibility. In this paper the following topics are discussed with emphasis: how the measurement error can be reduced, how the time scale of measurement can be extented without more occupation of the computer's memory, how Fo and Fi can be determined precisely in the fluorescence kinetics measurement by design and improvement of the software. The result presented in this paper indicated that the software and the home-made plant ms-range chlorophyll fluorescence kinetics photometer (PFM-101 ) can be widely used in the photosynthetic measurement and research in agriculcure, plant ecology and plant physiology.