植物学报 ›› 2007, Vol. 24 ›› Issue (05): 636-641.

• 组织培养简讯 • 上一篇    下一篇

芨芨草愈伤组织器官发生及植株再生

王瑛 周燕 高述民 梁蕊 刘艳 李莉 陈新德   

  1. 1 北京林业大学生命科学与技术学院, 北京 100083; 2 新疆石河子农八师农业局, 石河子 832000
  • 收稿日期:2006-09-27 修回日期:2007-02-13 出版日期:2007-09-01 发布日期:2007-09-01
  • 通讯作者: 高述民

In vitro Regeneration of Achnatherum splendens (Trin.) Nevski

Ying Wang Yan Zhou Shumin Gao Rui Liang Yan Liu Li Li Xinde Chen   

  1. 1College of Biological Science and Technology, Beijing Forestry University, Beijing 100083, China;2Agriculture Bureau of Xinjiang Shihezi Eighth Agriculture Division, Shihezi 832000, China
  • Received:2006-09-27 Revised:2007-02-13 Online:2007-09-01 Published:2007-09-01
  • Contact: Shumin Gao

摘要: 芨芨草(Achnatherum splendens (Trin.) Nevski)种子消毒并在MS培养基上萌发获得无菌苗, 以幼苗的叶鞘和胚轴为外植体诱导愈伤组织, 经继代后进一步诱导不定芽及生根。研究结果表明, 诱导愈伤组织最适合的培养基为B5+1.5 mg.L-12,4-D+0.5 mg.L-1 NAA; 诱导芽分化较适合的培养基为B5+0.5 mg.L-1 6-BA +0.2 mg.L-1 NAA; 1/4 B5+1.0 mg.L-1 NAA+0.2 mg.L-1 IBA +1.0 g.L-1活性炭培养基则有利于芨芨草试管苗的生根。本实验建立了完整的芨芨草植株再生体系, 移栽成活率高。

Abstract: We studied the regeneration of Achnatherum splendens (Trin.) Nevski in vitro and selected several favorable media.Germ-free plants were produced with germination on MS medium. Callus was induced with B5+1.5 mg.L-1 2,4-D+0.5 mg.L-1 NAA,with leaf sheath and hypocotyls as explants. After two subcultures, shoots and roots were induced. The medium containing B5+0.5 mg.L-1 6-BA + 0.2 mg.L-1 NAA was suitable for shoot induction. For root induction, the medium containing 1/4 B5+1.0 mg.L-1 NAA+ 0.2 mg.L-1 IBA +1.0 g.L-1 active carbon was favorable. With this regeneration system for A. splendens (Trin.) Nevski, we achieved the highest survival rate of transplants.