植物学报 ›› 2006, Vol. 23 ›› Issue (6): 708-717.

• 专题介绍 • 上一篇    下一篇

原子力显微镜在植物学研究中的应用

祖元刚 张宇亮 刘志国 王延兵 梁慧丽 刘红梅   

  1. 东北林业大学森林植物生态学教育部重点实验室, 哈尔滨 150040
  • 收稿日期:2006-06-13 修回日期:2006-08-26 出版日期:2006-11-20 发布日期:2006-11-20
  • 通讯作者: 祖元刚

Application of Atomic Force Microscope in Plant Biology Research

Yuangang Zu, Yuliang Zhang, Zhiguo Liu, Yanbing Wang, Huili Liang, Hongmei Liu   

  1. Key Laboratory of Forest Plant Ecology of Ministry of Education, Northeast Forestry University, Harbin 150040, China
  • Received:2006-06-13 Revised:2006-08-26 Online:2006-11-20 Published:2006-11-20

摘要: 原子力显微镜(AFM)是一种探测样品表面信息的有力工具, 它可以在空气和接近样品生理条件下成像, 同时也可以在皮牛(pico-Newton, 10-12 N)至微牛(micro-Newton, 10-6 N)水平上测量力的大小。本文主要介绍了自AFM发明以来, 其在植物大分子、细胞器、细胞、叶片等方面的应用, 并列举了目前 AFM存在的几点不足。

Abstract: Atomic force microscope (AFM) is a powerful tool for high-resolution imaging of the surface of samples under air or physiological conditions in measurements ranging from pico-Newtons (10-12 N) to micro-Newtons (10-6 N). In this paper, we discuss the potential application of AFM in plant biology research of, for example, macromolecules, organelles, cells, and leaves, and summarize the current limitations of the technique.