雌雄异株葡萄的性别鉴定研究

植物学报 ›› 1998, Vol. 15 ›› Issue (04): 63-67.

雌雄异株葡萄的性别鉴定研究

张立平林伯年沈德绪

1(浙江农业大学生物科学系　杭州　310029）　2（浙江农业大学园艺系　杭州　310029）

出版日期:1998-07-20 发布日期:1998-07-20
通讯作者: 张立平

The Study on Sexual Distinction of Dioecism in Vitis

ZHANG Li-Ping, LIN Bo-Nian and SHEN De-Xu

1(Department of Biology Science, Zhejiang Agricultural University, Hangzhou 310029) 2(Department of Horticulture, Zhejiang Agricultural University, Hangzhou 310029)

Online:1998-07-20 Published:1998-07-20
Contact: ZHANG Li-Ping

摘要/Abstract

摘要： 通过扫描电镜观察花粉的形态；分析叶片超氧化物歧化酶同工酶(SOD)；RAPD分析基因组DNA多态性，来鉴别葡萄的性别。结果表明：葡萄雌雄株的花粉形态存在着较大的差异；在本实验条件下葡萄雌雄株叶片SOD同工酶谱带一致；一些引物的RAPD产物间存在着多态性，在葡萄雌雄株的基因组DNA序列中存在着碱基排列顺序的差异。实验指出，花粉形态和基因组DNA的多态性分析是进行性别鉴定的有效方法。

Abstract: We differentiated the sexual distinction of dioecism Vitis by observing the shape of pollen under a scanning electron microscope; analyzing the isozyme of SOD in leaves; detecting the polymorphism of genomie DNA with the method of RAPD. The resulsts indicated that:there was a great difference in the pollen shape of male and female Vitis, the pattern of SOD isozyme were the same in male and female dioecism Vitis; some of the results of RAPD were polymorphic between the male and female. The shape of pollen and RAPD were effective for differentiating the sexual distinction of dioecism.

张立平林伯年沈德绪. 雌雄异株葡萄的性别鉴定研究. 植物学报, 1998, 15(04): 63-67.

ZHANG Li-Ping;LIN Bo-Nian and SHEN De-Xu. The Study on Sexual Distinction of Dioecism in Vitis. Chinese Bulletin of Botany, 1998, 15(04): 63-67.

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