植物学报 ›› 1996, Vol. 13 ›› Issue (02): 34-36.

• 研究报告 • 上一篇    下一篇

马铃薯转化效率的因素探讨

侯林林 李枞 观庆虎 孟淑兰 宋树德   

  1. (中国科学院植物研究所 北京 100093)
  • 出版日期:1996-03-20 发布日期:1996-03-20

Studies on the Potato Transformation Efficiency

Hou Lin-lin ,Li Cong, Ma Qing-hu, Meng Shu-lan and Song Yan-ru   

  1. (Institute of Botany, The Chinese Academy of Science, Beijing 100093)
  • Online:1996-03-20 Published:1996-03-20

摘要: 经农杆菌介导转化了5个马铃薯品种。结果表明不同品种的马铃薯转化效率不同试管薯度过2-3个月的休眠期后转化效率明显提高。转化效率亦随T-DNA长度的增加而减少,这可能与T-DNA整合时的断裂或重组有关。

Abstract: Five potato cultivars have been transferred by Agrobacterium. The results showed that the different cultivars have different transformation ratios. The transformation efficiency of the micro-tubers enhances significantly after 2-3 moths of dormancy. We also found that the increase of length of T-DNA decreases the transformation efficiency, this may be relative with split or rearrangement in T-DNA integration.