Abstract: We investigated in vitro culture and rapid propagation of the antituberculosis plant Chiritopsis repanda var. guilinensis. The optimal medium for primary culture for bud induction and seedling regeneration from leaves was MS+0.5 mg·L^–16-BA+0.05 mg·L^–1IBA (pH8.0). The optimal medium for subculture for bud multiplication was MS+0.1 mg·L^–16-BA+0.05 mg·L^–1IBA (pH6.0); the proliferation coefficient was 7.0/35 d. The optimal rooting medium was 1/2MS+0.2mg·L^–1NAA (pH6.0), and the rooting rate was 93.6%. The rooting plantlets were transplanted in a greenhouse that simulated the natural distribution environment of Chiritopsis repanda var. guilinensis in spring; the survival rate was more than 90%. According to this rapid propagation technique, about 460 000 seedlings were reproduced from a germfree plantlet in a year.