水杉愈伤组织诱导及植株再生

doi:10.3969/j.issn.1674-3466.2010.05.010

植物学报 ›› 2010, Vol. 45 ›› Issue (05): 604-608.DOI: 10.3969/j.issn.1674-3466.2010.05.010

水杉愈伤组织诱导及植株再生

黄翠¹, 景丹龙¹, 王玉兵¹, 范深厚², 陈发菊^1*

¹三峡大学生物技术研究中心, 宜昌 443002;
²湖北省利川市水杉原生母树管理站, 利川 445400

收稿日期:2009-10-09 修回日期:2010-03-25 出版日期:2010-09-01 发布日期:2010-09-20
通讯作者: 陈发菊

Callus Induction and Plant Regeneration of a Living Fossil, Metasequoia glyptostroboides

Cui Huang¹, Danlong Jing¹, Yubing Wang¹, Shenhou Fan², Faju Chen^1*

¹Biotechnology Research Center and Key Laboratory of Plant Natural Products of Hubei, China Three Gorges University,Yichang 443002, China;

²Hubei Lichuan Metasequoia Mother Trees Administrative Station, Lichuan 445400, China

Received:2009-10-09 Revised:2010-03-25 Online:2010-09-01 Published:2010-09-20
Contact: Faju Chen

摘要/Abstract

摘要： 通过愈伤组织诱导器官发生途径, 建立了水杉(Metasequoia glyptostroboides)的植株再生体系, 探讨了不同外植体 (种胚、幼叶切块、茎段、根段)和植物生长调节剂对不定芽直接再生和愈伤组织诱导器官发生的影响。结果表明: 以种胚、无菌苗叶片、茎段和根作为外植体, 在MS补加2,4-D、NAA和6-BA不同组合的培养基上都能诱导得到愈伤组织, 其中种胚诱导愈伤组织效果最好, 诱导率可达100%, 茎诱导效果次之, 诱导率为97.1%。诱导愈伤组织效果较好的培养基有:MS+1.0 mg·L^–1 2,4-D + 0.5 mg·L^–1 6-BA、MS + 0.1 mg·L^–1 6-BA + 1.0 mg·L^–1 NAA、MS + 0.5 mg·L^–1 6-BA+1.0 mg·L^–1 NAA、MS+1.0 mg·L^–1 6-BA+1.0 mg·L^–1 NAA、MS+0.5 mg·L^–1 6-BA+2.0 mg·L^–1 NAA、MS+1.0 mg·L^–1 6-BA + 2.0 mg·L^–1 NAA和MS + 0.5 mg·L^–1 2,4-D + 0.5 mg·L^–1 NAA。以愈伤组织在MS培养基上植株再生效果最好, 再生率为62.5%。

Abstract: We optimized a rapid, effective system of plant regeneration by direct shoot regeneration and organogenesis in an endangered tree, the living fossil Metasequoia glyptostroboides. We studied the influence of plant growth regulators on explant types (embryo, root, stem and young leaf). Callus could be induced by different types of explants on MS medium supplemented with different combinations of 2,4-D, NAA and 6-BA. The frequency of callus induction was highest (100%) with embryo used as explants, then with stem (97.1%). The best media for callus induction on embryo was MS+1.0 mg·L^–1 2,4-D+0.5 mg·L^–1 6-BA, MS+0.1 mg·L^–1 6-BA+1.0 mg·L^–1 NAA, MS+0.5 mg·L^–1 6-BA+1.0 mg·L^–1 NAA, MS+1.0 mg·L^–1 6-BA+1.0 mg·L^–1 NAA, MS+0.5 mg·L^–1 6-BA+2.0 mg·L^–1 NAA, MS+1.0 mg·L^–1 6-BA+2.0 mg·L^–1 NAA and MS+0.5 mg·L^–1 2,4-D+0.5 mg·L^–1 NAA. More regenerated plants were obtained on MS medium, with a frequency of 62.5%.

黄翠, 景丹龙, 王玉兵, 范深厚, 陈发菊. 水杉愈伤组织诱导及植株再生. 植物学报, 2010, 45(05): 604-608.

Cui Huang, Danlong Jing, Yubing Wang, Shenhou Fan, Faju Chen. Callus Induction and Plant Regeneration of a Living Fossil, Metasequoia glyptostroboides. Chinese Bulletin of Botany, 2010, 45(05): 604-608.

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水杉愈伤组织诱导及植株再生

Callus Induction and Plant Regeneration of a Living Fossil, Metasequoia glyptostroboides

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