组培增殖方式对网纹草嵌合性状稳定性的影响

doi:10.3724/SP.J.1259.2015.00372

植物学报 ›› 2015, Vol. 50 ›› Issue (3): 337-338.DOI: 10.3724/SP.J.1259.2015.00372

组培增殖方式对网纹草嵌合性状稳定性的影响

王燕, 汪一婷, 吕永平, 牟豪杰, 李海营, 陈剑平^*()

浙江省农业科学院病毒与生物技术研究所, 农业部植保生物技术重点实验室, 杭州 310021

收稿日期:2014-04-15 接受日期:2014-08-25 出版日期:2015-05-01 发布日期:2015-04-08
通讯作者: 陈剑平
作者简介:
? 共同第一作者
基金资助:
引进国际先进农业科学技术计划(948项目) (No.2011-G31)和中国博士后科学基金(No.2012M521202)

Studies of the Chimeric Stability of Fittonia albivenis with Different Proliferation Methods of Tissue Culture

Yan Wang, Yiting Wang, Yongping Lü, Haojie Mou, Haiying Li, Jianping Chen^*

Ministry of Agriculture Key Laboratory of Biotechnology in Plant Protection, Institute of Virus and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2014-04-15 Accepted:2014-08-25 Online:2015-05-01 Published:2015-04-08
Contact: Chen Jianping
About author:
? These authors contributed equally to this paper

摘要/Abstract

摘要： 以一种具有良好的谱系细胞标记的周缘嵌合体网纹草(Fittonia albivenis)为研究材料, 对不同增殖方式下其组培苗嵌合性状的稳定性进行了观察分析。结果表明, 以茎段诱导腋芽增殖时网纹草的稳定性很高, 但以丛生芽增殖时其嵌合性状发生了变异, 变异率为21.32%; 叶片组织解剖学观察结果显示, 母本嵌合体茎尖分生组织中红和绿2种细胞谱系的细胞层排布在丛生芽再生过程中发生了改变, 这可能是导致新类型嵌合体产生的原因。研究结果不仅对植物嵌合体种苗的组培生产具有重要的指导意义, 而且为嵌合体的种质创新工作提供了新方法。

Abstract: The chimeric stability of Fittonia albivenis with different proliferation methods was analysed, with assistance from favourable markers of cell lineage. The axillary bud proliferation was appropriate for the multiplication of ornamental chimera plants; the variation rate of cluster-bud proliferation was 21.32%, and the two lineages with red and green color separately were found rearranged in the shoot apical meristem of cluster buds, which might directly induce the appearance of new chimera types. This study offers insights into the production of chimera plantlets via tissue culture and a new method for producing innovative germplasm for plant chimeras.

王燕, 汪一婷, 吕永平, 牟豪杰, 李海营, 陈剑平. 组培增殖方式对网纹草嵌合性状稳定性的影响. 植物学报, 2015, 50(3): 337-338.

Yan Wang, Yiting Wang, Yongping Lü, Haojie Mou, Haiying Li, Jianping Chen. Studies of the Chimeric Stability of Fittonia albivenis with Different Proliferation Methods of Tissue Culture. Chinese Bulletin of Botany, 2015, 50(3): 337-338.

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图/表 4

表1 不同增殖方式对网纹草增殖率和稳定性的影响

Table 1 Effects of different multiplication ways on the proliferation rate and stabilities of Fittonia albivenis

Proliferation way	No. of explants	No. of buds propagated	No. of buds variated	Proliferation rate	Variation rate (%)
Axillary bud proliferation	64	182	2	2.84±0.06 b	1.10±0.32 b
Cluster bud proliferation	64	272	58	4.30±0.20 a	21.32±2.77 a

图1 丛生芽增殖过程中产生的新类型网纹草的叶片形态 (A) 母本类型; (B) 新类型1; (C) 新类型2; (D) 新类型3。Bar=1 mm

Figure 1 The leaf morphology of the new Fittonia albivenis types generated by cluster-bud proliferation (A) Mother plant; (B) New type 1; (C) New type 2; (D) New type 3. Bar=1 mm

图2 Induction rate of different Fittonia albivenis types during cluster bud proliferation

Figure 2

图3 不同类型网纹草嵌合体的叶片解剖学观察(左侧为叶缘部位) (A) 母本类型; (B) 新类型1; (C) 新类型2; (D) 新类型3。Bar=100 μm

Figure 3 Leaf histological structures of different Fittonia albivenis types (The left side is leaf margin) (A) Mother plant; (B) New type 1; (C) New type 2; (D) New type 3. Bar=100 μm

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组培增殖方式对网纹草嵌合性状稳定性的影响

Studies of the Chimeric Stability of Fittonia albivenis with Different Proliferation Methods of Tissue Culture

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