禾本科牧草和草坪草雄核发育与单倍体育种研究进展

doi:10.3724/SP.J.1259.2012.00087

摘要/Abstract

摘要： 禾本科牧草与草坪草在农业可持续发展、城市绿化和生态环境保护方面起着至关重要的作用。近年来, 随着生物技术的发展, 国内外在牧草及草坪草雄核发育与单倍体育种研究方面取得了较大进展。该文在归纳总结该领域研究成果的基础上, 对影响禾本科牧草及草坪草雄核发育与单倍体育种的几个主要因素进行了探讨。大量研究结果表明, 供试材料的基因型是影响培养效率的最主要因素。小孢子发育到单核中期至晚期时有利于提高培养效率。培养前花药经过低温和甘露醇等预处理不但可以提高愈伤组织的诱导效率, 还可提高愈伤组织的质量。适宜的激素种类和配比也是影响培养成败的关键因素。同时, 总结了雄核发育再生植株的倍性鉴定方法和加倍技术, 对单倍体育种技术在禾本科牧草及草坪草育种中的应用前景进行了展望。

Abstract: Gramineous forage and turfgrass are critical to sustainable agriculture and contribute extensively to urban landscaping and ecological protection. In recent years, with the rapid development of biotechnology, significant progress has been made in androgenesis and haploid breeding research of gramineous forage and turfgrasses. This review describes the development of androgenesis systems for gramineous forage and turfgrass and summarizes the latest progress of some important influencing factors on androgenesis and haploid breeding. The genotype of experimental materials is the main factor influencing androgenesis efficiency. The optimal stage for efficient androgenesis of microspore development is between middle and late uninucleate. The ratio and quality of callus induction were improved after anthers were pretreated with low temperature or mannitol. Suitable varieties and ratios of hormone are also the key factors in androgenesis. We summarize the methods of ploidy determination and techniques of chromosome doubling and point out prospects for further research into double haploid breeding in forage and turfgrass.

赵永钦, 吴新新, 李蔚, 李仁, 史文君, 郭仰东. 禾本科牧草和草坪草雄核发育与单倍体育种研究进展. 植物学报, 2012, 47(1): 87-99.

Yongqin Zhao, Xinxin Wu, Wei Li, Ren Li, Wenjun Shi, Yangdong Guo. Research Progress in Androgenesis and Haploid Breeding of Gramineous Forage and Turfgrass Plants. Chinese Bulletin of Botany, 2012, 47(1): 87-99.

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://www.chinbullbotany.com/CN/10.3724/SP.J.1259.2012.00087

https://www.chinbullbotany.com/CN/Y2012/V47/I1/87

参考文献

曹禹, 李鹏飞, 甘玲玉, 孙娜, 魏凌基 (2007). 冬性小黑麦花药培养的基因型和培养基效应的研究. 新疆农业科学 44, 91 － 100.
董静, 邹飞波, 张国平 (2005). 麦类作物小孢子培养研究进展. 麦类作物学报 25, 102 － 106.
杜永芹, 陈如梅, 张国荣, 余立云, 黄培忠, 陆雪琴 (2001). 天然麦芽提取液对大麦花药培养力的影响. 上海农业学报 17, 27 － 30.
杜永芹, 陈如梅, 张国荣, 奏国卫, 余立云, 马俊虎, 黄培忠 (1997). 气温、ABT等因素对大麦花药离体培养的影响. 上海农业学报 13, 19 － 22.
郭向荣, 方红曼, 李安生, 胡含 (1997). 甘露醇预处理方式、方法对大麦花粉植株再生的影响. 农业生物技术学报 7, 321 － 324.
韩彬, 陈孝, 徐惠君, 张文祥, 辛志勇, 黄惠宇(1990). 硬粒小麦-簇毛麦双单倍体愈伤组织染色体加倍技术的研究. 植物学报 32, 191 － 197.
韩福光, 张颖 (1993). 高粱不同外植体愈伤组织诱导的研究. 辽宁农业科学 1, 45 － 48.
黄斌 (1985). 大麦花药培养中低温预处理对花粉愈伤组织形成的影响. 植物学报 27, 439 － 443.
李守岭, 庄南生 (2006). 植物花药培养及其影响因素研究进展(综述). 亚热带植物科学 35, 76 － 80.
李文泽, 胡含 (1991). 大麦花药培养中甘露醇预处理作用的研究. 莱阳农学院学报 8, 252 － 256.
李文泽, 胡含 (1995). 不同预处理方法对大麦花药-花粉培养的影响. 遗传 17, 5 － 7.
李志武, 徐惠君, 叶兴国(1996). 小麦花药培养中染色体加倍技术作物杂志. 4, 24 － 26.
马艳红, 于卓, 李小雷, 李造哲(2006). 加拿大披碱草与2种国产披碱草杂种F1愈伤组织诱导及植株再生研究. 西北植物学报26, 1888 － 1892.
马艳红, 于卓, 李小雷, 刘杰, 李造哲(2007). 加拿大披碱草×圆柱披碱草杂种F1的生育及细胞遗传学分析. 麦类作物学报27, 45 － 48.
萨日娜, 陈永胜, 黄凤兰, 李国瑞, 姚远 (2008). 植物花药培养技术研究进展. 内蒙古民族大学学报 23, 650 － 653.
孙敬三, 路铁刚, 吴逸, 李守全 (1991). 大麦花药培养技术的改进. 植物学通报 8, 27 － 29.
孙娜, 石培春, 魏凌基, 曹禹, 甘铃玉, 李鹏飞 (2009). 冬春性小黑麦花药愈伤组织诱导的影响因素. 石河子大学学报 27, 1 － 5.
孙月芳, 陆瑞菊 (2003). 大麦优穗和油菜花蕾提取液对大麦花药培养反应的影响. 麦类作物学 23, 19 － 22.
王子霞, 张茂银, 杨克锐, 海热古力?阿布力孜, 杨松杰, 顾爱松, 戚家华 (2000). 新疆大赖草幼胚花药培养及植株再生. 新疆农业科学 4, 159 － 161.
袁惠燕, 姜茜, 张珙(2008). 花粉植株染色体倍性及加倍技术研究进展. 北方园艺 2, 61－64.
袁妙葆, 朱睦元, 王林济, 潘伟槐, 徐阿炳 (1989). 大麦花药培养中不同基因型和不同培养基条件对出愈率和分化率的效应研究. 科技通报 3, 52 － 55.
朱建华, 彭士勇 (2001). 植物组织培养实用技术. 北京: 中国计量出版社, pp. 106 － 107.
Abdullah AA, Pedersen S, Andersen SB (1994). Triploid and hexaploid regenerants from hexaploid timothy (Phleum pratense L.) via anther culture. Plant Breed 112, 342 － 345.
Arabi MLE, Al-Safadi B, Jawhar M, Mir-Alin N (2005). Enhancement of embryogenesis and plant regeneration from barley anther culture by low doses of gamma irradiation. In Vitro Cell.Dev.Biol.-Plant 41, 762 － 764.
Boppenmeier J, Züchner S, Foroughi-Wehr B (1989). Haploid production from barley yellow dwarf virus resistant clones of Lolium. Plant Breed 103, 216 － 220.
Chekurov VM, Razmakhnin EP (1999). Effect of inbreeding and growth regulators on the in vitro androgenesis of wheatgrass, Agropyron glaucum. Plant Breed 118, 571 － 573.
Christensen JR, Borrino E, Olesen A, Andersen SB (1997). Diploid, tetraploid, and octoploid plants from anther culture of tetraploid orchardgrass, Dactylis glomerata L. Plant Breed 116, 261 － 270.
Cistué L, Ramos A, Castillo AM (1999). Influence of anther pretreatment and culture medium composition on the production of barley doubled haploids from model and low responding cultivars. Plant Cell, Tissue and Organ Cult 55, 159 － 166.
Dainel G (1993). Anther culture in rye: improvement plant regeneration using modified MS-media. Plant Breed 110, 259 － 261.
Davies PA, Morton SA (1998). A comparison of barley isolated microspore and anther culture and the influence of cell culture density. Plant Cell Rep 17, 206 － 210.
Day A, Ellis THN (1984). Chloroplast DNA deletions associated with wheat plant regeneration from pollen: possible basis for maternal inheritance of chloroplasts. Cell 39, 359 － 368.
Day A, Ellis THN (1985). Deleted forms of plastid DNA in albino plants from cereal tissue culture. Curr Gen 9, 671 － 678.
Dunford R, Walden RM (1991). Plastid genome structure and plastid-related transcript levels in albino barley plants derived from anther culture. Curr Genet 20, 339 － 347.
Dunwell JM, Francis RJ, Powell W (1987). Anther culture of Hordeum vulgare L.: a genetic study of microspore callus production and differentiation. Theor Appl Genet 74, 60 － 64.
Ferrie AMR, Palmer CE, Keller WA (1995). Haploid embryogenesis. In: Thorpe TA (ed.). In Vitro Embryogenesis in Plants. London, Kluwer Academic Publishers. pp. 309-344.
Flehinghaus-Roux T, Deimling S, Geiger HH (1995) Anther culture ability in Secale cereale L. Plant Breed 114, 259 － 261.
Flehinghaus T, Deimling S,Deimling S, Geiger H (1991). Methodical improvements in rye anther culture. Plant Cell Rep 10, 397 － 400.
Foroughi-Wehr B, Friedt W. Wenzel W (1982). On the Genetic Improvement of Androgenetic Haploid Formationin Hordeum vulgate L Theor Appl Genet 62, 233 － 239
Guha S, Maheshwari SC (1964). In vitro production of embryos from anthers of datura. Nature, 204, pp. 476.
Guo,Y-D, Hisano H , Shimamoto Y (2009) Transformation of androgenic-derived Festulolium plants (Lolium perenne L.× Festuca pratensis Huds.)by Agrobacterium tumefaciens. Plant Cell Tissue and Organ Cult 96, 219 － 227.
Guo Y-D , Mizukami Y, Yamada T (2005). Genetic characterization of androgenic progeny derived from Lolium perenne x Festuca pratensis cultivars. New Phytologist 166, 455 － 464.
Guo Y-D, Pulli S (2000). Isolated microspore culture and plant regeneration in rye (Secale cereale L.). Plant Cell Rep 19, 875 － 880.
Guo Y-D, Pulli S (2000). An efficient androgenic embryogenesis and plant regeneration method through isolated microspore culture in timothy(Phleum pratense L.). Plant Cell Rep 19,761 － 767.
Guo Y-D, Sewón P, Pulli S (1999). Improved embryogenesis from anther culture and plant regeneration in timothy. Plant Cell, Tissue and Organ Cult 57, 85 － 93.
Halberg N, Olesen A, Tuvesson IKD (1990). Genotypes of perennial ryegrass (Lolium perenne L.) with high anther culture response through hybridization. Plant Breed 105, 89 － 94.
Harada T, Sato T, Asaka D, Matsukawa I (1991). Large-scale deletions of rice plastid DNA in anther culture. Theor Appl Genet 81,157 － 161.
Haydu Z, Vasil. IK (1981). Somatic Embryogenesis and Plant Regeneration from Leaf Tissues and Anthers of Pennisetum purpureum Schum. Theor. Appl. Genet 59, 269 － 273.
Hoekstra S, Zijderveld MH, Louwerse JD, Heidekamp F, vander Mark (1992). Anther and microspore culture of Hordeum vulgare L. cv. Igri. Plant Sci 86, 89 － 96.
Immonen S, Anntila H (1996). Success in anther culture of rye. Vortr. Plfanzenzüchtg 35, 237 － 244.
Immonen S, Anntila H (2000). Media composition and anther plating for production of androgenic green plants from cultivated rye (Secale cereale L.). Plant Physiol 156, 204 － 210.
Jacquard C, Asakaviciute R, Hamalian AM (2006). Barley anther culture: effects of annual cycle and spike position on microspore embryogenesis and albinism. Plant Cell Rep , 25, 375 － 381.
J?hne A, L?rz H (1995). Cereal microspore culture. Plant Sci, 109,1 － 12. K?hler F , Wenzel G (1985). Regeneration of isolated barley microspores in conditioned media and trials to characterise the responsible factor. Plant Physiol 121, 181 － 191.
Karsai I, Bed? Z, Hayes P.M (1994). Effect of induction medium pH and maltose concentration on in vitro androgenesis of hexaploid winter triticale and wheat .Plant Cell, Tissue Organ Cult 39, 49 － 53.
Kasperbauer M J, Eizenga G C (1985). Tall fescue doubled haploids via tissue culture and plant regeneration [J]. Crop Sci 25, 1091 － 1095.
Kiviharju E, Moisander S, Laurila J (2005). Improved green plant regeneration rates from oat anther culture and the agronomic performance of some DH lines. Plant Cell, Tissue and Organ Cult 81, 1 － 9.
Kiviharju E, Pehu E (1998). The effect of cold and heat pretreatments on anther culture response of Avena sativa and A Sterilis. Plant Cell Tissue Organ Cult 54, 97 － 104.
Kiviharju E, Puolimatka M, Pehu E (1997). Regeneration of anther-derived plants of Avena sterilis. Plant Cell Tissue Organ Cult 48, 147 － 152.
Kiviharju E, Tauriainen AA (1999). 2,4-Dichlorophenoxyacetic acid and kinetin in anther culture of cultivated and wild oats and their interspecific crosses: plant regeneration from A. sativa L. Plant Cell Rep 18, 582 － 588.
Kumaravadivel N, Rangsamy SR (1994). Plant regeneration from sorghum anther cultures and field evaluation of progeny. Plant Cell Rep 13, 286 － 290.
Lazaridou TB, Lithourgidia AS, Kotzamanidis ST (2005). Anther culture response of barley genotypes to cold pretreatments and culture media. Russ J Plant Physiol 52, 696 － 699.
Madsen S, Olsen A, Dennis B, Andersen SB (1995). Inheritance of anther-culture response in Perennial ryegrass. Plant Breed 114, 165 － 168.
MaheshWari SC, Tyagi AK, Malhotra K (1980). Induction of haploidy from pollen grains in angiosperms the current status. Theor Appl Genet 58, 193 － 206.
Marciniak K, Banaszak Z., Wedzony M.(1998). Effect of genotype, medium and sugar on triticale(Triticosecale Wittm.) anther culture response. Cereal Res Commun 26, 145 － 151.
Ma R, Guo Y-D, Pulli S (2004). Comparison of anther and microspore culture in the embryogenesis and regeneration of rye (Secale cereale). Plant Cell, Tissue and Organ Cult 76, 147 － 157.
Niizeki M, Kati F (1973). Studies on plant cell and tissue culture .Ⅲ.In vitro production of callus from anther culture of forage crops. Fac.Agric.Hokkaido Univ 57, 293 － 300.
Nitsch C, Norreel B (1973). Effect dum choctechnique surpepouvoir embrygnose due pollende Datura innoxia cultive dansl'anther ouisolede anthere. C.R.Acad.Sci.Paris 276, 303 － 306.
Olesen A, Andersen SB, Deu IK (1988). Anther Culture Response in Perennial Ryegrass (Lolium perenne L.) Plant Breed 101, 60 － 65.
Opsahl-Ferstad HG, Bjornstad A, Rognli OA (1994). Influence of medium and cold pretreatment on androgenetic response in Lolium perenne L. Plant Cell Rep 13, 594 － 600.
Park CH,Walton PD (1989). Embryo-callus-regenerated hybrids and their colchicines induced amphiploids between Elymus canadenisi and Secale cereale. Theor Appl Genet 78, 721 － 727 .
Parminder K, Sidhu, Philip A. Davies (2009). Regeneration of fertile green plants from oat isolated microspore culture. Plant Cell Rep 28, 571 － 577
Ponitka A, ?lusarkiewicz-Jarzina A.(2007). The Effect of Liquid and Solid Medium on Production of Winter Triticale (×Triticosecale Wittm.)Anther-Derived Embryos and Plants. Cereal Res Commun 35, 15 － 22.
Reynolds TL,Crawford RL (1996). Changes in abundance of an abscisic acid-responsive early cysteine labeled metallothionein transcript during pollen embryogenesis in bread wheat(Triticum aestivum) [J]. Plant Mo1 Bio1 32, 823 － 829.
Richard RC, Wang, Joy E, Marburger, Chen JH (1991). Tissue-culture-facilitated production of aneupolyhaploid Thinopyrum ponticum and amphidiploid Hordeum violaceum ×H. bogdanii and their uses in phylogenetic studies. Theor Appl Gene 81, 151 － 156.
Rines HW (1983). Oat anther culture: genotype effect on callus initiation and the production of a haploid plant. Crop Sci 23, 268 － 272.
Rose JB, Dunwell JM, Sunderland N (1986). Anther culture of Sorghum bicolor L. Moench: Effect of panicle pretreament, anther incubation temperature and 2,4-D concentration. Plant Cell Tiss Organ Cult 6, 15 － 22.
Rose JB, Dunwell JM, Sunderland N (1987). Anther Culture of Lolium temulentum, Festuca pratensis and Lolium ×Festuca Hybrids. Influence of Pretreatment, Culture Medium and Culture Incubation Conditions on Callus Production and Differentiation. Annals of Botany 60, 191 － 201.
Shigemune A, Yoshida T (2000). Methods of anther culture of pearl millet and ploidy level of regenerated plants. J J Crop Sci 69, 227 － 228.
?lusarkiewicz-Jarzina A. Ponitka A (1997). Effect of genotype and media composition on embryoid induction and plant regeneration from anther culture in triticale. J Appl Genet 38, 252 － 258.
Thomas E, Hoffmann F, Wenzel G (1975) Haploid plantlets from microspores of rye. Z.Pflanzenzüchtg 75, 106 － 113.
Thomas E, Wenzel G (1975). Embryogenesis from microspores of rye. Naturwissenschaften, 62, 40 － 41.
Tuvesson S, Ljungberg A, Johansson N, Karlsson KE, Suus LW, Josset JP (2000). Large-scale production of wheat and triticale double haploids through the use of a single-anther culture method. Plant Breed 119, 455-459.
Vrinten PL,Nakamura T,Kasha KJ (1999). Characterization of cDNAs expressed in the early stages of microspo re embryogenesis in barley (Hordeum vulgre L)[J]. Plant Mo1 Bio1 41, 455 － 463.
Wen FS, Sorensen EL, Barnett FL (1991). Callus induction and regeneration from anther and inflorescence culture of Sorghum. Euphytica 52, 177 － 181.
Wenzel G, Hoffmann F, Potrykus I, Thomas E (1975). The separation of viable rye microspores from mixed populations and their development in culture. Mol Gen Genet 138, 293 － 297.
Wenzel G, Hoffmann F, Thomas E (1976). Heterozygous microspore-derived plants in rye. Theor App Genet 48, 205 － 208.
Wenzel G, Hoffmann F, Thomas E (1977). Increased induction and chromosome doubling of and rogenetic haploid rye. Theor Appl Genet 51, 81 － 86.
Wenzel G, Thomas E (1974). Observation of the growth in culture of anthers of Secale cereale L. Z. Pflanzenzüchtg 72, 89 － 94.
Yao K, Chen CH, McMullen CR. (1991) Regeneration of albino shoots in cultured anthers of intermediate wheatgrass(Agropyron intermedium (Host) Beauv.). Plant Cell, Tissue and Organ Cult 26,189 － 193.
Zare AG, Humphreys MW, Rogers JW, Mortimer AM, Collin HA (2002). Androgenesis in a Lolium multiflorum × Festuca arundinacea hybrid to generate genotypic variation for drought resistance, Euphytica 125,1 － 11.
Zwierzykowski Z, Zwierzykowska E, Slusarkiewicz-Jarzina A, Ponitka A (1999). Regeneration of anther-derived plants from pentaploid hybrids of Festuca arundinacea× Lolium multiflorum. Euphytica 105, 191 － 195.