水稻类Tubby蛋白质在叶片生长和白叶枯病抗性反应中的表达

doi:10.3724/SP.J.1259.2011.00525

植物学报 ›› 2011, Vol. 46 ›› Issue (5): 525-533.DOI: 10.3724/SP.J.1259.2011.00525

水稻类Tubby蛋白质在叶片生长和白叶枯病抗性反应中的表达

魏健¹, 李莉云¹, 曹英豪^1,2, 刘雨萌¹, 巩校东¹, 刘丽娟¹, 张园园¹, 刘国振^1*

¹河北农业大学生命科学学院, 保定 071001;
²中国科学院北京基因组研究所, 北京 101318

收稿日期:2011-01-18 修回日期:2011-04-13 出版日期:2011-09-01 发布日期:2011-09-01
通讯作者: 刘国振
基金资助:
国家自然科学基金青年基金项目;国家自然科学基金青年基金项目

Expression of Tubby-like Proteins in Rice Leaves and During Interactions Between Rice and Xanthomonas oryzae pv. oryzae

Jian Wei¹, Liyun Li¹, Yinghao Cao^1,2, Yumeng Liu¹, Xiaodong Gong¹, Lijuan Liu¹, Yuanyuan Zhang¹, Guozhen Liu^1*

¹College of Life Sciences, Hebei Agricultural University, Baoding 071001, China

²Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 101318, China

Received:2011-01-18 Revised:2011-04-13 Online:2011-09-01 Published:2011-09-01
Contact: Guozhen Liu

摘要/Abstract

摘要： 类Tubby蛋白质(Tubby-like protein, TLP)在动植物中广泛存在, 暗示其在生命过程中发挥重要的作用。水稻(Oryza sativa)基因组中有14个TLP家族成员, 首先制备了这些蛋白质的抗体, 用免疫印迹方法检测了它们在水稻叶片不同生长时期的表达情况, 揭示其表达模式; 然后对Xa21介导的水稻白叶枯病抗性反应不同时间点进行检测, 发现OsTLP2、OsTLP7、OsTLP8和OsTLP9等4个蛋白质的表达发生了变化; 进一步比较它们在抗病、感病反应和对照处理中的表达情况, 发现不同反应间的表达也有区别。该研究结果为阐释水稻TLP在叶片生长过程中的功能, 尤其是在水稻-白叶枯病菌互作过程中的作用提供了重要线索。

Abstract: The universal existence of tubby-like proteins (TLPs) both in animals and plants implies that TLPs may play key roles in basic biological processes. In rice genome there are 14 members of TLP genes. We examined the expression of TLPs in rice leaves at different developmental stages with TLP-specific antibodies. We also investigated the expression pattern of TLPs at different times in incompatible responses mediated by bacterial blight-resistant gene Xa21 against Xanthomonas oryzae pv. oryzae (Xoo). We found that the expression of OsTLP2, 7, 8 and 9 varied in incompatible responses and that the expression patterns varied among compatible interaction, incompatible interaction and mock control. These results provide important clues for the interpretation of TLP function in rice, especially in the interaction between rice and Xoo.

魏健, 李莉云, 曹英豪, 刘雨萌, 巩校东, 刘丽娟, 张园园, 刘国振. 水稻类Tubby蛋白质在叶片生长和白叶枯病抗性反应中的表达. 植物学报, 2011, 46(5): 525-533.

Jian Wei, Liyun Li, Yinghao Cao, Yumeng Liu, Xiaodong Gong, Lijuan Liu, Yuanyuan Zhang, Guozhen Liu. Expression of Tubby-like Proteins in Rice Leaves and During Interactions Between Rice and Xanthomonas oryzae pv. oryzae. Chinese Bulletin of Botany, 2011, 46(5): 525-533.

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水稻类Tubby蛋白质在叶片生长和白叶枯病抗性反应中的表达

Expression of Tubby-like Proteins in Rice Leaves and During Interactions Between Rice and Xanthomonas oryzae pv. oryzae

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