植物学报

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植物接头蛋白AP复合体的研究进展

王玉同1, 2, 3†, 田丹5†, 费琼晖1, 2, 3†, 李旭泰1, 严旭4, 赵菲佚1, 2*, 徐梅4*   

  1. 1天水师范学院生物工程与技术学院, 天水 741001; 2天水师范学院硫生物技术研究所, 天水 741001; 3甘肃省农业固体废弃物资源化利用重点实验室, 天水 741001; 4绍兴文理学院生命与环境科学学院, 绍兴 312000; 5华南农业大学生命科学学院, 广州 510642


  • 收稿日期:2025-02-02 修回日期:2025-03-26 出版日期:2025-05-21 发布日期:2025-05-21
  • 通讯作者: 赵菲佚, 徐梅
  • 基金资助:
    国家自然科学基金(No.32200607)、甘肃省科技计划(No.24JRRE005, No.24JRRE007)、甘肃省教育厅高校教师创新基金(No.2024A-115)和浙江省自然科学基金(No.LQ23C160005)

Research Advances of AP Complexes in Plants

Yutong Wang1, 2, 3, Dan Tian5, Qionghui Fei1, 2, 3, Xutai Li1, Xu Yan4, Feiyi Zhao1, 2*, Mei Xu4*   

  1. 1School of Bioengineering and Biotechnology, Tianshui Normal University, Tianshui 741001; 2Institute of Sulfur Biotechnology, Tianshui Normal University, Tianshui 741001; 3Gansu Key Laboratory of Utilization of Agricultural Solid Waste Resources, Tianshui 741001; 4School of Life and Environmental Sciences, Shaoxing University, Shaoxing 312000; 5College of Life Sciences, South China Agricultural University, Guangzhou 510642

  • Received:2025-02-02 Revised:2025-03-26 Online:2025-05-21 Published:2025-05-21

摘要: 膜蛋白运输(membrane protein trafficking)是真核细胞生命活动中最重要的事件之一, 在细胞分裂、分化、生长、胞外营养物质吸收、胞内外信号传递、逆境防御等细胞生物学过程中具有重要功能。膜蛋白运输主要包括胞吞(endocytosis)、胞吐(exocytosis)和液泡/溶酶体降解(vacuolar/lysosomal degradation)等囊泡运输(vesicular trafficking)途径, 而接头蛋白(adaptor protein, AP)在膜受体蛋白识别、货物分拣(cargo sorting)、网格蛋白的招募、装配和拆卸, 以及辅助蛋白的招募等囊泡运输过程中具有重要调控功能。目前, 真核生物中已经发现5种AP复合体, 分别是AP-1、AP-2、AP-3、AP-4和AP-5。本文以动物及酵母细胞AP研究进展为基础, 主要对植物细胞AP复合体的组成、结构、亚细胞定位及其生物学功能进行了综述。并对AP复合体中需要进一步研究的重要科学问题进行了探讨。

关键词: 膜蛋白运输, 囊泡运输, 接头蛋白复合体, 植物

Abstract: Membrane protein trafficking is one of the most important events of eukaryotic cells, which plays a crucial role in cell biology processes such as cell division, differentiation, growth, extracellular nutrient absorption, intracellular signal transduction, and stress defense. Membrane protein transport mainly involves vesicular trafficking pathways including endocytosis, exocytosis, and vacuolar/lysosomal degradation. Adaptor proteins (APs) play an important regulatory role in vesicular trafficking processes such as recognition of membrane receptor proteins, cargo sorting, recruitment and assembly of clathrin, and recruitment of accessory proteins. Currently, five AP complexes have been identified in eukaryotic organisms, namely AP-1, AP-2, AP-3, AP-4, and AP-5. This article mainly reviews the composition, structure, subcellular localization, and biological functions of the plant AP complexes based on the research progress of animal and yeast cell AP. Finally, we initinal some questions about potential function of AP complex and present future perspectives in plants.

Key words: membrane protein trafficking, vesicle trafficking, adaptor protein complexes, plant