植物学报

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植物接头蛋白AP复合体的研究进展

王玉同1,田丹2,费琼晖1,李旭泰1,严旭3,赵菲佚1,徐梅3   

  1. 1. 天水师范学院
    2. 华南农业大学
    3. 绍兴文理学院
  • 收稿日期:2025-02-02 修回日期:2025-03-26 出版日期:2025-05-21 发布日期:2025-05-21
  • 通讯作者: 徐梅
  • 基金资助:
    网格蛋白和接头蛋白复合体AP-4调控植物自噬的机理研究;乙烯信号调控植物网格蛋白质膜招募的机制研究;接头蛋白AP-MUTANT调控植物育性的分子机理研 究;探究地膜覆盖对玉米网格蛋白介导营养元素吸收的影响;TgWRI1调控香榧油脂合成的分子机制

Research Advances of AP Complexes in Plants

Yu-tong WANG1, Fei-YI ZHAO3,   

  • Received:2025-02-02 Revised:2025-03-26 Online:2025-05-21 Published:2025-05-21

摘要: 膜蛋白运输(membrane protein trafficking)是真核细胞生命活动中最重要的事件之一,在细胞分裂、分化、生长、胞外营养物质吸收、胞内外信号传递、逆境防御等细胞生物学过程中具有重要功能。膜蛋白运输主要包括胞吞(endocytosis)、胞吐(exocytosis)和液泡/溶酶体降解(vacuolar/lysosomal degradation)等囊泡运输(vesicle trafficking)途径,而接头蛋白(Adaptor protein, AP)在膜受体蛋白识别、货物分拣(cargo sorting)、网格蛋白的招募、装配和拆卸,以及辅助蛋白的招募等囊泡运输过程中具有重要调控功能。目前,真核生物中已经发现了5种AP复合体,分别是AP-1、AP-2、AP-3、AP-4和AP-5。本文以动物及酵母细胞AP研究进展为基础,主要对植物细胞AP复合体的组成、结构、亚细胞定位及其生物学功能进行了综述。并对 AP复合体研究中需要进一步研究的的重要科学问题进行了探讨。

关键词: 膜蛋白运输, 囊泡运输, 接头蛋白复合体, 植物

Abstract: Membrane protein trafficking is one of the most important events of eukaryotic cells, which plays a crucial role in cell biology processes such as cell division, differentiation, growth, extracellular nutrient absorption, intracellular and extracellular signaling, and stress defense. Membrane protein transport mainly involves vesicle trafficking pathways including endocytosis, exocytosis, and vacuolar/lysosomal degradation. Adaptor proteins (APs) play an important regulatory role in vesicle trafficking processes such as recognition of membrane receptor proteins, cargo sorting, recruitment and assembly of clathrin, and recruitment of auxiliary proteins. Currently, five AP complexes have been identified in eukaryotic organisms, namely AP-1, AP-2, AP-3, AP-4, and AP-5. This article mainly reviews the composition, structure, subcellular localization, and biological functions of the plant AP complexes based on the research progress of animal and yeast cell AP. Finally, we initinal some questions about potential function of AP complex and present future perspectives in plants.

Key words: membrane protein trafficking, vesicle trafficking, adaptor protein complexes, plant