植物学报

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贝莱斯芽孢杆菌对芒果炭疽病的抑制作用及果实保鲜效果

曹慧1, 羊尾燕2, 纳琦婷1, 朱长松1, 孟兰环1, 宋海超2, 史学群2*   

  1. 1海南大学食品科学与工程学院, 海口570228; 2海南大学生命健康学院, 海口570228
  • 收稿日期:2025-01-15 修回日期:2025-02-06 出版日期:2025-02-26 发布日期:2025-02-26
  • 通讯作者: 史学群

The Inhibitory Efficacy of Bacillus velezensis Against Mango Anthracnose and Its Influence on Fruit Fresh Preservation

Hui Cao1, Weiyan Yang2, Qiting Na1, Changsong Zhu1, Lanhuan Meng1, Haichao Song2, Xuequn Shi2*   

  1. 1College of Food Science and Engineering, Hainan University, Haikou 570228, China; 2College of Life and Health Sciences, Hainan University, Haikou 570228, China
  • Received:2025-01-15 Revised:2025-02-06 Online:2025-02-26 Published:2025-02-26
  • Contact: Xuequn Shi

摘要: 炭疽病是芒果主要病害之一, 是由胶孢炭疽菌(Colletotrichum gloeosporioides)侵染引起。发病后影响果实品质从而缩短果实货架期。该研究从生物防治角度出发, 以贝莱斯芽孢杆菌(Bacillus velezensis)和从芒果果实中分离出的炭疽菌进行实验, 用贝莱斯芽孢杆菌菌悬液(CB)处理芒果果实, 并利用平板对峙法观察CB对胶孢炭疽菌的抑制效果。通过发酵液(CF)处理胶孢炭疽菌, 结果表明发酵液能够有效抑制胶孢炭疽菌的孢子萌发并缩短芽管长度, 显著抑制菌丝生长, 其中发酵液(CF)浓度为2%和4%时, 对炭疽菌菌丝抑制率分别为75.18%和80.96%。果实活体接种实验表明, CB (菌悬液)和CF (发酵液)能有效抑制果实病斑直径的增大, 抑制率分别为44.33%和65.00%; 与对照组相比, 处理组可溶性固形物含量显著降低, 减缓了可滴定酸含量的降低, 对果实品质未造成负面影响; 同时也延缓了果实总酚和类黄酮含量的降低。实验表明, 贝莱斯芽孢杆菌菌株能够抑制芒果炭疽病的发生, 在芒果保鲜方面有较大的应用潜力。

关键词: 芒果, 胶孢炭疽菌, 贝莱斯芽孢杆菌, 生物防治, 果实保鲜

Abstract: INTRODUCTION: Anthracnose, primarily caused by Colletotrichum gloeosporioides is a main diseaseaffecting mangoes, leading to significant postharvest losses by deteriorating fruit quality and reducing shelf life.  RATIONALE: Addressing postharvest anthracnose is a critical challenge in the mango industry. Biological control methods, such as utilizing antagonistic bacteria, offer sus-tainable alternatives to chemical treatments. This study investigates the efficacy of Ba-cillus velezensis in inhibiting C. gloeosporioides and its potential in preserving mango fruit quality.  RESULTS: The application of B. velezensis culture filtrate (CF) effectively inhibited spore germination and mycelial growth of C. gloeosporioides. At CF concentrations of 2% and 4%, mycelial inhibition rates were 75.18% and 80.96%, respectively. In vivo experiments demonstrated that both bacterial suspension (CB) and CF treatments significantly re-duced lesion expansion on mangoes, with inhibition rates of 44.33% and 65.00%, respectively. Treated fruits exhibited a slower decrease in titratable acids and maintained higher levels of total phenols and flavonoids, indicating delayed ripening and extended shelf life.   CONCLUSION: Bacillus velezensis exhibits strong antagonistic activity against C. gloeosporioides, effectively controlling mango anthracnose and preserving fruit quality. Its application as a biocontrol agent holds promise for sustainable postharvest management in mango production.

Key words: Mango, Colletotrichum gloeosporioides, Bacillus velezensis, biological control, fresh preservation