植物sirtuin蛋白家族研究进展

doi:10.11983/CBB22255

摘要/Abstract

摘要： 植物sirtuin蛋白家族是一类依赖β-烟酰胺腺嘌呤二核苷酸(β-NAD⁺)、分别催化非组蛋白和组蛋白赖氨酸上N^ε-乙酰基和N^ε-酰基(乙酰基、巴豆酰基、丁酰基和2-羟基异丁酰基)去除的酶, 具有调控非组蛋白活性和基因表达的功能。已证明sirtuin蛋白家族成员在水稻(Oryza sativa)、大豆(Glycine max)和番茄(Solanum lycopersicum)等植物的生长发育以及盐、冷、热和病原菌等胁迫响应中发挥重要的表观调控作用。该文对植物sirtuins的酶活性、催化底物、细胞定位和功能进行综述, 为理解其表观遗传调控机制及丰富新功能研究提供参考。

关键词: 植物, sirtuins, 去酰基, 表观遗传调控, 发育, 胁迫

Abstract: The sirtuin protein family of plants is a class of enzymes that depend on β-nicotinamide adenine dinucleotide (β-NAD⁺) to catalyze the removal of N^ε-acetyl and N^ε-acyl (acetyl, crotonyl, butylyl and 2-hydroxy-isobutylyl) from non-histone and histone lysine, respectively, and has the function of regulating the activity of non-histone protein and gene expression. Members of the sirtuin protein family have been shown to play important epigentic regulatory roles in the growth and development of rice (Oryza sativa), soybean (Glycine max) and tomato (Solanum lycopersicum), and in response to salt, cold, heat and pathogen stresses. Here we summarized the recent research advances in the enzyme activity, catalytic substrates, cell localization and function of plant sirtuins, which will serve as a reference for understanding the epigenetic regulation mechanism and studying the new function of sirtuin.

Key words: plant, sirtuins, deacylation, epigenetic regulation, development, stress

苏鲁方, 王萍, 李顺, 蔡燕, 郭丹丹, 刘琴, 刘小云. 植物sirtuin蛋白家族研究进展. 植物学报, 2023, 58(6): 998-1007.

Lufang Su, Ping Wang, Shun Li, Yan Cai, Dandan Guo, Qin Liu, Xiaoyun Liu. Research Progress in Sirtuin Protein Family in Plants. Chinese Bulletin of Botany, 2023, 58(6): 998-1007.

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB22255

https://www.chinbullbotany.com/CN/Y2023/V58/I6/998

图/表 3

参考文献 55

[1]	陈聪, 宋江波, 孟刚, 童晓玲, 代方银, 鲁成 (2014). 家蚕sirtuin家族基因的鉴定及系统发生与表达芯片分析. 中国农业科学 47, 2659-2670. DOI
[2]	陈威, 杨颖增, 陈锋, 周文冠, 舒凯 (2019). 表观遗传修饰介导的植物胁迫记忆. 植物学报 54, 779-785. DOI
[3]	Bheda P, Jing H, Wolberger C, Lin HN (2016). The substrate specificity of sirtuins. Annu Rev Biochem 85, 405-429. DOI PMID
[4]	Blander G, Guarente L (2004). The Sir2 family of protein deacetylases. Annu Rev Biochem 73, 417-435. PMID
[5]	Bourque S, Dutartre A, Hammoudi V, Blanc S, Dahan J, Jeandroz S, Pichereaux C, Rossignol M, Wendehenne D (2011). Type-2 histone deacetylases as new regulators of elicitor-induced cell death in plants. New Phytol 192, 127-139. DOI PMID
[6]	Bruscalupi G, Di Micco P, Failla CM, Pascarella G, Morea V, Saliola M, De Paolis A, Venditti S, Mauro ML (2023). Arabidopsis thaliana sirtuins control proliferation and glutamate dehydrogenase activity. Plant Physiol Biochem 194, 236-245. DOI URL
[7]	Cantó C, Auwerx J (2011). NAD⁺ as a signaling molecule modulating metabolism. Cold Spring Harb Symp Quant Biol 76, 291-298. DOI PMID
[8]	Carabetta VJ, Cristea IM (2017). Regulation, function, and detection of protein acetylation in bacteria. J Bacteriol 199, e00107-17.
[9]	Carrico C, Meyer JG, He WJ, Gibson BW, Verdin E (2018). The mitochondrial acylome emerges: proteomics, regulation by sirtuins, and metabolic and disease implications. Cell Metab 27, 497-512. DOI PMID
[10]	Chen B, Zang WW, Wang J, Huang YJ, He YH, Yan LL, Liu JJ, Zheng WP (2015). The chemical biology of sirtuins. Chem Soc Rev 44, 5246-5264. DOI PMID
[11]	Chen XY, Xu QT, Duan YH, Liu H, Chen XL, Huang JB, Luo CX, Zhou DX, Zheng L (2021). Ustilaginoidea virens modulates lysine 2-hydroxyisobutyrylation in rice flowers during infection. J Integr Plant Biol 63, 1801-1814. DOI URL
[12]	Chen Z, Luo L, Chen RF, Hu HH, Pan YF, Jiang HB, Wan X, Jin H, Gong YM (2018). Acetylome profiling reveals extensive lysine acetylation of the fatty acid metabolism pathway in the diatom Phaeodactylum tricornutum. Mol Cell Proteomics 17, 399-412. DOI URL
[13]	Chu JS, Chen Z (2018). Molecular identification of histone acetyltransferases and deacetylases in lower plant Marchantia polymorpha. Plant Physiol Biochem 132, 612-622. DOI URL
[14]	Chung PJ, Kim YS, Park SH, Nahm BH, Kim JK (2009). Subcellular localization of rice histone deacetylases in organelles. FEBS Let 583, 2249-2254. DOI URL
[15]	Cucurachi M, Busconi M, Morreale G, Zanetti A, Bavaresco L, Fogher C (2012). Characterization and differential expression analysis of complete coding sequences of Vitis vinifera L. sirtuin genes. Plant Physiol Biochem 54, 123-132. DOI URL
[16]	De Block M, Van Lijsebettens M (2011). Energy efficiency and energy homeostasis as genetic and epigenetic components of plant performance and crop productivity. Curr Opin Plant Biol 14, 275-282. DOI PMID
[17]	De Block M, Verduyn C, De Brouwer D, Cornelissen M (2005). Poly (ADP-ribose) polymerase in plants affects energy homeostasis, cell death and stress tolerance. Plant J 41, 95-106. PMID
[18]	Dutta A, Abmayr SM, Workman JL (2016). Diverse activities of histone acylations connect metabolism to chromatin function. Mol Cell 63, 547-552. DOI PMID
[19]	Fan W, Luo JY (2010). SIRT1 regulates UV-induced DNA repair through deacetylating XPA. Mol Cell 39, 247-258. DOI PMID
[20]	Fang CY, Zhang H, Wan J, Wu YY, Li K, Jin C, Chen W, Wang SC, Wang WS, Zhang HW, Zhang P, Zhang F, Qu LH, Liu XQ, Zhou DX, Luo J (2016). Control of leaf senescence by an MeOH-jasmonates cascade that is epigenetically regulated by OsSRT1 in rice. Mol Plant 9, 1366-1378. DOI URL
[21]	Fang Y, Tang S, Li XL (2019). Sirtuins in metabolic and epigenetic regulation of stem cells. Trends Endocrinol Metabol 30, 177-188. DOI URL
[22]	Feldman JL, Dittenhafer-Reed KE, Denu JM (2012). Sirtuin catalysis and regulation. J Biol Chem 287, 42419-42427. DOI PMID
[23]	Greiss S, Gartner A (2009). Sirtuin/Sir2 phylogeny, evolutionary considerations and structural conservation. Mol Cells 28, 407-415. DOI PMID
[24]	Guarente L (2011). The logic linking protein acetylation and metabolism. Cell Metab 14, 151-153. DOI PMID
[25]	Hashida SN, Takahashi H, Uchimiya H (2009). The role of NAD biosynthesis in plant development and stress responses. Ann Bot 103, 819-824. DOI URL
[26]	Huang LM, Sun QW, Qin FJ, Li C, Zhao Y, Zhou DX (2007). Down-regulation of a SILENT INFORMATION REGULATOR2-related histone deacetylase gene, OsSRT1, induces DNA fragmentation and cell death in rice. Plant Physiol 144, 1508-1519. DOI URL
[27]	Imai SI, Armstrong CM, Kaeberlein M, Guarente L (2000). Transcriptional silencing and longevity protein Sir2 is an NAD-dependent histone deacetylase. Nature 403, 795-800. DOI
[28]	Kaelin WG Jr, McKnight SL (2013). Influence of metabolism on epigenetics and disease. Cell 153, 56-69. DOI PMID
[29]	Kang M, Abdelmageed H, Lee S, Reichert A, Mysore KS, Allen RD (2013). AtMBP-1, an alternative translation product of LOS2, affects abscisic acid responses and is modulated by the E3 ubiquitin ligase AtSAP5. Plant J 76, 481-493. DOI URL
[30]	König AC, Hartl M, Pham PA, Laxa M, Boersema PJ, Orwat A, Kalitventseva I, Plöchinger M, Braun HP, Leister D, Mann M, Wachter A, Fernie AR, Finkemeier I (2014). The Arabidopsis class II sirtuin is a lysine deacetylase and interacts with mitochondrial energy metabolism. Plant Physiol 164, 1401-1414. DOI URL
[31]	Lee IH, Cao L, Mostoslavsky R, Lombard DB, Liu J, Bruns NE, Tsokos M, Alt FW, Finkel T (2008). A role for the NAD-dependent deacetylase Sirt1 in the regulation of autophagy. Proc Natl Acad Sci USA 105, 3374-3379.
[32]	Lee K, Park OS, Jung SJ, Seo PJ (2016). Histone deacetylation-mediated cellular dedifferentiation in Arabidopsis. J Plant Physiol 191, 95-100. DOI URL
[33]	Li SC, Zheng WP (2018). Mammalian sirtuins SIRT4 and SIRT7. Prog Mol Biol Transl Sci 154, 147-168.
[34]	Liu XY, Wei W, Zhu WJ, Su LF, Xiong ZY, Zhou M, Zheng Y, Zhou DX (2017). Histone deacetylase AtSRT1 links metabolic flux and stress response in Arabidopsis. Mol Plant 10, 1510-1522. DOI URL
[35]	Lu Y, Xu QT, Liu Y, Yu Y, Cheng ZY, Zhao Y, Zhou DX (2018). Dynamics and functional interplay of histone lysine butyrylation, crotonylation, and acetylation in rice under starvation and submergence. Genome Biol 19, 144. DOI PMID
[36]	Narita T, Weinert BT, Choudhary C (2019). Functions and mechanisms of non-histone protein acetylation. Nat Rev Mol Cell Biol 20, 156-174. DOI
[37]	O'Callaghan C, Vassilopoulos A (2017). Sirtuins at the crossroads of stemness, aging, and cancer. Aging Cell 16, 1208-1218. DOI PMID
[38]	Rajabi N, Galleano I, Madsen AS, Olsen CA (2018). Targeting sirtuins: substrate specificity and inhibitor design. Prog Mol Biol Transl Sci 154, 25-69.
[39]	Reed SM, Quelle DE (2015). p53 acetylation: regulation and consequences. Cancers 7, 30-69. DOI URL
[40]	Ringel AE, Tucker SA, Haigis MC (2018). Chemical and physiological features of mitochondrial acylation. Mol Cell 72, 610-624. DOI PMID
[41]	Sridha S, Wu KQ (2006). Identification of AtHD2C as a novel regulator of abscisic acid responses in Arabidopsis. Plant J 46, 124-133. DOI URL
[42]	Sundaresan NR, Pillai VB, Wolfgeher D, Samant S, Vasudevan P, Parekh V, Raghuraman H, Cunningham JM, Gupta M, Gupta MP (2011). The deacetylase SIRT1 promotes membrane localization and activation of AKT and PDK1 during tumorigenesis and cardiac hypertrophy. Sci Signal 4, ra46.
[43]	Tang WS, Zhong L, Ding QQ, Dou YN, Li WW, Xu ZS, Zhou YB, Chen J, Chen M, Ma YZ (2022). Histone deacetylase AtSRT2 regulates salt tolerance during seed germination via repression of vesicle-associated membrane protein 714 (VAMP714) in Arabidopsis. New Phytol 234, 1278-1293. DOI URL
[44]	Vall-Llaura N, Torres R, Lindo-García V, Muñoz P, Munné-Bosch S, Larrigaudière C, Teixidó N, Giné- Bordonaba J (2021). PbSRT1 and PbSRT2 regulate pear growth and ripening yet displaying a species-specific regulation in comparison to other Rosaceae spp. Plant Sci 308, 110925. DOI URL
[45]	Wang CZ, Gao F, Wu JG, Dai JL, Wei H, Li Y (2010). Arabidopsis putative deacetylase AtSRT2 regulates basal defense by suppressing PAD4, EDS5 and SID2 expression. Plant Cell Physiol 51, 1291-1299. DOI URL
[46]	Yamamori T, DeRicco J, Naqvi A, Hoffman TA, Mattagajasingh I, Kasuno K, Jung SB, Kim CS, Irani K (2010). SIRT1 deacetylates APE1 and regulates cellular base excision repair. Nucleic Acids Res 38, 832-845. DOI PMID
[47]	Yang C, Shen WJ, Chen HF, Chu LT, Xu YC, Zhou XC, Liu CL, Chen CM, Zeng JH, Liu J, Li QF, Gao CJ, Charron JB, Luo M (2018). Characterization and subcellular localization of histone deacetylases and their roles in response to abiotic stresses in soybean. BMC Plant Biol 18, 226. DOI PMID
[48]	Zhang F, Wang LK, Ko EE, Shao K, Qiao H (2018). Histone deacetylases SRT1 and SRT2 interact with ENAP1 to mediate ethylene-induced transcriptional repression. Plant Cell 30, 153-166. DOI URL
[49]	Zhang H, Lu Y, Zhao Y, Zhou DX (2016). OsSRT1 is involved in rice seed development through regulation of starch metabolism gene expression. Plant Sci 248, 28-36. DOI PMID
[50]	Zhang H, Zhao Y, Zhou DX (2017). Rice NAD⁺-dependent histone deacetylase OsSRT1 represses glycolysis and regulates the moonlighting function of GAPDH as a transcriptional activator of glycolytic genes. Nucleic Acids Res 45, 12241-12255. DOI PMID
[51]	Zhao LM, Lu JX, Zhang JX, Wu PY, Yang SG, Wu KQ (2015). Identification and characterization of histone deacetylases in tomato (Solanum lycopersicum). Front Plant Sci 5, 760.
[52]	Zhao S, Zhang XR, Li HT (2018). Beyond histone acetylation-writing and erasing histone acylations. Curr Opin Struct Biol 53, 169-177. DOI URL
[53]	Zhao SM, Xu W, Jiang WQ, Yu W, Lin Y, Zhang TF, Yao J, Zhou L, Zeng YX, Li H, Li YX, Shi J, An WL, Hancock SM, He FC, Qin LX, Chin J, Yang PY, Chen X, Lei QY, Xiong Y, Guan KL (2010). Regulation of cellular metabolism by protein lysine acetylation. Science 327, 1000-1004. DOI PMID
[54]	Zheng WP (2020). Review: the plant sirtuins. Plant Sci 293, 110434. DOI URL
[55]	Zhong XC, Zhang H, Zhao Y, Sun QW, Hu YF, Peng H, Zhou DX (2013). The rice NAD⁺-dependent histone deacetylase OsSRT1 targets preferentially to stress- and metabolism-related genes and transposable elements. PLoS One 8, e66807. DOI URL

物种	蛋白名称	细胞定位	催化底物	酶活性	功能	参考文献
拟南芥 (Arabidopsis thaliana)	AtSRT1	细胞核	H3K9ac和AtMBP1	去乙酰基	非生物胁迫响应和叶片发育	Lee et al., 2016; Liu et al., 2017
	AtSRT2A	线粒体	ATP合酶和AAC1/2/3	去乙酰基	能量代谢	K?nig et al., 2014
	AtSRT2B	细胞核	H4K8	去乙酰基	盐胁迫响应	Tang et al., 2022
		细胞核	组蛋白和H3K9ac	去乙酰基	生长发育和病原菌胁迫响应	Wang et al., 2010
		线粒体	ATP合酶和AAC1/2/3	去乙酰基	能量代谢	K?nig et al., 2014
水稻 (Oryza sativa)	OsSRT1	细胞核	H3K9ac、OsGAPDH1/ 2/5和Khib	去乙酰基和去2-羟基异丁酰基	叶片衰老、种子发育、能量代谢和病原菌胁迫响应	Zhong et al., 2013; Fang et al., 2016; Zhang et al., 2016, 2017
	OsSRT2	细胞质	OsGAPDH1	去乙酰基	能量代谢	Lu et al., 2018
		线粒体	H3K9ac	去乙酰基	能量代谢	Chung et al., 2009; Lu et al., 2018
		细胞核	组蛋白和H3K9	去乙酰基、去巴豆酰基、去丁酰基和去2-羟基异丁酰基	黑暗、饥饿、淹水和病原菌胁迫响应	Lu et al., 2018; Chen et al., 2021
大豆 (Glycine max)	GmSRT1	细胞质和线粒体	H3组蛋白	去乙酰基	-	Yang et al., 2018
	GmSRT2	细胞质和线粒体	-	-	冷、热和盐胁迫响应	Yang et al., 2018
	GmSRT3	细胞质和细胞核	-	-	-	Yang et al., 2018
	GmSRT4	细胞核	-	-	冷、热和盐胁迫响应	Yang et al., 2018
番茄(Solanum lycopersicum)	SlSRT1	细胞核	-	-	子叶、花和果实发育	Zhao et al., 2015
番茄(Solanum lycopersicum)	SlSRT2	细胞核和细胞质	-	-	子叶、叶、花和果实发育	Zhao et al., 2015
葡萄(Vitis vinifera)	VvSRT1	细胞核和细胞质	-	-	根、叶、花和果实发育	Cucurachi et al., 2012
葡萄(Vitis vinifera)	VvSRT2	细胞质和线粒体	-	-	叶、花和果实发育	Cucurachi et al., 2012
地线 (Marchantia polymorpha)	MpSIRT4	线粒体	-	-	IAA和热胁迫响应	Chu and Chen, 2018
	MpSIRT5	线粒体	-	-	ABA响应	Chu and Chen, 2018
	MpSIRT6	细胞核和细胞质	-	-	-	Chu and Chen, 2018
三角褐指藻 (Phaeodacty- lum tricornutum)	PtSRT5	质体和细胞核	PtACSL1	去乙酰基	脂肪酸合成	Chen et al., 2018
	PtSRT3	-	-	-	-	Chen et al., 2018
	PtSRT2	-	-	-	-	Chen et al., 2018
	PtSRT4	-	-	-	-	Chen et al., 2018

物种	蛋白名称	细胞定位	催化底物	酶活性	功能	参考文献
拟南芥 (Arabidopsis thaliana)	AtSRT1	细胞核	H3K9ac和AtMBP1	去乙酰基	非生物胁迫响应和叶片发育	Lee et al., 2016; Liu et al., 2017
	AtSRT2A	线粒体	ATP合酶和AAC1/2/3	去乙酰基	能量代谢	K?nig et al., 2014
	AtSRT2B	细胞核	H4K8	去乙酰基	盐胁迫响应	Tang et al., 2022
		细胞核	组蛋白和H3K9ac	去乙酰基	生长发育和病原菌胁迫响应	Wang et al., 2010
		线粒体	ATP合酶和AAC1/2/3	去乙酰基	能量代谢	K?nig et al., 2014
水稻 (Oryza sativa)	OsSRT1	细胞核	H3K9ac、OsGAPDH1/ 2/5和Khib	去乙酰基和去2-羟基异丁酰基	叶片衰老、种子发育、能量代谢和病原菌胁迫响应	Zhong et al., 2013; Fang et al., 2016; Zhang et al., 2016, 2017
	OsSRT2	细胞质	OsGAPDH1	去乙酰基	能量代谢	Lu et al., 2018
		线粒体	H3K9ac	去乙酰基	能量代谢	Chung et al., 2009; Lu et al., 2018
		细胞核	组蛋白和H3K9	去乙酰基、去巴豆酰基、去丁酰基和去2-羟基异丁酰基	黑暗、饥饿、淹水和病原菌胁迫响应	Lu et al., 2018; Chen et al., 2021
大豆 (Glycine max)	GmSRT1	细胞质和线粒体	H3组蛋白	去乙酰基	-	Yang et al., 2018
	GmSRT2	细胞质和线粒体	-	-	冷、热和盐胁迫响应	Yang et al., 2018
	GmSRT3	细胞质和细胞核	-	-	-	Yang et al., 2018
	GmSRT4	细胞核	-	-	冷、热和盐胁迫响应	Yang et al., 2018
番茄(Solanum lycopersicum)	SlSRT1	细胞核	-	-	子叶、花和果实发育	Zhao et al., 2015
番茄(Solanum lycopersicum)	SlSRT2	细胞核和细胞质	-	-	子叶、叶、花和果实发育	Zhao et al., 2015
葡萄(Vitis vinifera)	VvSRT1	细胞核和细胞质	-	-	根、叶、花和果实发育	Cucurachi et al., 2012
葡萄(Vitis vinifera)	VvSRT2	细胞质和线粒体	-	-	叶、花和果实发育	Cucurachi et al., 2012
地线 (Marchantia polymorpha)	MpSIRT4	线粒体	-	-	IAA和热胁迫响应	Chu and Chen, 2018
	MpSIRT5	线粒体	-	-	ABA响应	Chu and Chen, 2018
	MpSIRT6	细胞核和细胞质	-	-	-	Chu and Chen, 2018
三角褐指藻 (Phaeodacty- lum tricornutum)	PtSRT5	质体和细胞核	PtACSL1	去乙酰基	脂肪酸合成	Chen et al., 2018
	PtSRT3	-	-	-	-	Chen et al., 2018
	PtSRT2	-	-	-	-	Chen et al., 2018
	PtSRT4	-	-	-	-	Chen et al., 2018