植物学报 ›› 2017, Vol. 52 ›› Issue (1): 30-42.DOI: 10.11983/CBB16107

所属专题: 水稻生物学专辑

• 研究论文 • 上一篇    下一篇

水稻主栽品种空育131抗稻瘟病位点的扫描及其基因组重构建

张晓慧, 冯晓敏, 林少扬*   

  1. 中国科学院遗传与发育生物学研究所, 北京 100101
  • 收稿日期:2016-05-11 接受日期:2016-06-15 出版日期:2017-01-01 发布日期:2017-01-23
  • 通讯作者: 林少扬
  • 作者简介:

    # 共同第一作者

  • 基金资助:
    中国科学院分子模块设计育种创新体系先导专项(No.XDA08030102)

Scanning for Pi Loci and Rebuilding an Improved Genome of Elite Rice Variety Kongyu 131

Xiaohui Zhang, Xiaomin Feng, Shaoyang Lin*   

  1. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101
  • Received:2016-05-11 Accepted:2016-06-15 Online:2017-01-01 Published:2017-01-23
  • Contact: Lin Shaoyang
  • About author:

    # Co-first authors

摘要:

空育131粳稻(Oryza sativa ssp. japonica)品种因具有早熟质优、丰产稳产及耐低温冷害等优点成为黑龙江省的第一大主栽品种。为了挽救其近年来由于感染稻瘟病而从生产上退出的局面, 通过对主栽品种空育131基因组的重测序和扫描, 明确其遗缺多个优良抗稻瘟病Pi基因(Pi2Pi9Pi36Pi5-1Pb1Pid3Pi25PikhPi1Pik-mPik-pPi56t等), 并通过回交育种的方法, 将MP水稻材料中的Pb1广谱抗瘟基因片段导入空育131染色体组中。该基因组的再构建过程尽可能不改变原品种的其它优良性状, 并利用控制目标导入片段长短的策略来缩短Pb1位点附近的连锁累赘。在目前得到的导入系中, 目标导入片段长约700 kb, 背景回复率为99.38%。表型鉴定结果显示, 该导入系可能和亲本MP水稻材料发挥同等的抗瘟能力。

Abstract:

The rice variety Kongyu 131 (Oryza sativa ssp. japonica) is the most widely grown elite cultivar in Heilongjiang province because of its high quality, early maturity, high yield and cold resistance. However, because it has been cultivated in the same areas for many years, it is now highly susceptible to rice blast. By re-sequencing and scanning the whole genome of Kongyu 131, we found that it lacks more than 12 cloned blast resistance genes, including Pi2, Pi9, Pi36, Pi5-1, Pb1, Pid3, Pi25, Pikh, Pi1, Pik-m, Pik-p and Pi56t. To improve the blast resistance, we successfully introgressed Pb1 (Panicle blast 1) into Kongyu131. Without changing the other agronomic characters, we used 5 single nucleotide polymorphism markers to control the length of the introduced target fragment derived from the donor cultivar MP. Inoculating test results indicated that the improved line, containing a 700 kb target fragment and sharing 99.38% genetic background with Kongyu 131, showed the same blast resistance as MP.