植物学报 ›› 2017, Vol. 52 ›› Issue (1): 43-53.DOI: 10.11983/CBB16081

所属专题: 水稻生物学专辑

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水稻非生物胁迫响应基因OsMIP1的表达与进化分析

王玲1,2, 郭长奎1,2,3, 任丁1,2,*(), 马红1,2,*()   

  1. 1复旦大学生命科学学院, 上海 200438
    2复旦大学植物科学研究所, 上海 200438
    3浙江农林大学农业与食品科学学院, 杭州 311300
  • 收稿日期:2016-04-16 接受日期:2016-05-26 出版日期:2017-01-01 发布日期:2017-01-23
  • 通讯作者: 任丁,马红
  • 作者简介:

    # 共同第一作者

  • 基金资助:
    转基因生物新品种培育(No.2014ZX08009-014B);转基因生物新品种培育(No.2014ZX08009-014B)

Molecular Evolution and Expression Analysis of the OsMIP1 Response to Abiotic Stress

Wang Ling1,2, Guo Changkui1,2,3, Ren Ding1,2,*(), Ma Hong1,2,*()   

  1. 1School of Life Sciences, Fudan University, Shanghai 200438, China
    2Institute of Plant Biology, Fudan University, Shanghai 200438, China
    3School of Agriculture and Food Science, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China
  • Received:2016-04-16 Accepted:2016-05-26 Online:2017-01-01 Published:2017-01-23
  • Contact: Ren Ding,Ma Hong
  • About author:

    # Co-first authors

摘要:

MID1编码R-R型的MYB转录因子, 对不同的非生物胁迫均有响应, 特别是在水稻(Oryza sativa)生殖期会受到干旱胁迫的诱导, 进而在一定程度上可以保持花粉的育性并稳定水稻产量。为进一步研究水稻MID1对非生物胁迫的响应网络, 利用酵母双杂交系统筛选出与其互作的蛋白因子OsMIP1, 并利用双分子荧光互补系统在本氏烟草(Nicotiana benthamiana)细胞中得到验证。结果表明, OsMIP1编码1个预测含有ENTH/ANTH/VHS结构域的跨膜转运蛋白。OsMIP1在根、茎、叶、小穗和胚乳中均有表达。干旱胁迫下, OsMIP1在叶片和生殖器官中表达, 特别是在减数分裂后的小花中表达显著上调。这些结果暗示, OsMIP1在花器官抵抗干旱胁迫中起一定的作用。在水稻营养生长阶段, OsMIP1表达还受到包括NaCl和甘露醇在内的其它非生物胁迫的影响, 暗示其可能在其它非生物胁迫调节中也具有一定的作用。植物中关于编码ENTH/ ANTH/VHS结构域蛋白的研究很少。通过对MIP1亚家族进化关系进行分析, 结果表明, 在被子植物中, MIP1可分为6大类, 这6大类分别来自被子植物祖先中原本就存在的6个拷贝, 在被子植物的进化过程中又经历了多次基因重复和拷贝丢失等事件。MIP1家族成员广泛分布于被子植物中并可能具有抗胁迫等功能。

Abstract:

The gene MID1 (MYB IMPORTANT FOR DROUGHT RESPONSE1) encodes a putative R-R type MYB transcription factor; is induced by abiotic stresses, especially drought in reproductive stage; and can improve pollen fertility and rice production. To understand the role of MID1 in abiotic stress responses, we used the yeast two-hybrid system to find an interacting protein, OsMIP1 (Oryza sativa MID1 interaction protein 1). The interaction was further confirmed by BiFC (bimolecular fluorescence complementation) analysis in tobacco leaf cells. OsMIP1 encodes a putative transmembrane protein with an ENTH/ANTH/VHS domain. It is expressed in the root, stem, leaf, panicle and endosperm. Under drought stress, its expression is upregulated in leaf and reproductive organs, especially in post-meiotic flowers. OsMIP1 may play a role in response to drought stress during reproductive development. OsMIP1 expression during vegetative development can be induced by other abiotic stress, including NaCl and mannitol, which suggests that OsMIP1 can respond to other abiotic stresses. There is little analysis of the evolution of genes encoding proteins with the ENTH/ ANTH/VHS domain, so we analyzed the molecular evolution of MIP1 homologs in flowering plants. The evolution analysis of the MIP1 family in angiosperms showed that MIP1 homologs can be divided into 6 types, which originated from at least 6 copies of MIP1 homologous genes in the ancestor of extent angiosperms. After gene-duplication and -loss events, MIP1 family members widely distributed in the angiosperms and might have various functions, possibly in stress responses.