Chin Bull Bot ›› 2012, Vol. 47 ›› Issue (4): 405-412.doi: 10.3724/SP.J.1259.2012.00405

Previous Articles     Next Articles

Changes of Pigments in Sabina przewalskii and Picea crassifolia Needles Along with Different Altitudes

Longying Wen1,2, Tuo Chen2*   

  1. 1College of Chemistry and Life Sciences, Leshan Normal University, Leshan 614004, China

    2State Key Laboratory of Cryospheric Sciences, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China
  • Received:2011-07-22 Revised:2012-04-18 Online:2012-07-26 Published:2012-07-01
  • Contact: Tuo Chen

Abstract: Using high performance liquid chromatography (HPLC) and microplate spectrophotometer, we determined variations in pigments and enzymes phenylalanine ammonialyase (PAL) and UDP glucose-flavonoid 3-O-glucosyl-transferase (UFGT) involved in biosynthesis pathway of anthocyanins in Sabina przewalskii and Picea crassifolia along with altitude changes. The content of pigments and enzyme activity were dependent on the altitude: the anthocyanins (Acy) increased between 3 100–3 200 m, decreased with the increase of altitude in general. The enzyme activity of UFGT was increased with the increase of altitude while it fluctuated around 3 000 m. The enzyme activity of PAL in S. przewalskii was increased with the increase of altitude and the same pattern appeared in P. crassifolia between 2 800–3 000 m. The enzyme activity of PAL in P. crassifolia was decreased with the increase of altitude above 3 000 m. Contents of rhodoxanthin (Rhd) and carotenoids (Car), de-epoxidation index (A+Z)/(V+A+Z) were higher with higher altitude. The content of chlorophyll (Chl) had an opposite pattern: decreased with increasing altitude. Except for chlorophyll and PAL, the needles of S. przewalskii contained higher pigments than those of P. crassifolia. It was noted that the level of UFGT activity in S. przewalskii was twice that in P. crassifolia. Plants would encounter various environmental stresses caused by different altitude and season. Drought and strong sunlight are dominant in August for two species of Qilian Mountain. These pigments are mainly for anti-drought and prevent strong sunlight. Therefore, the role of pigments depends on when and where these plants inhabit.

CLC Number: 

  • Q945

[1] Baozhu Li, Xiaoliang Zhao, Lei Peng. Research Advances in the Development and Regulation of Plant Chloroplasts [J]. Chin Bull Bot, 2014, 49(3): 337-345.
[2] Xueqin Wei, Changfeng Zhang, Jie Pang, Dandan Kou, Run Lan. Research Progress in the Chemistry and Structure of Plant Cuticles Related to Water Permeability [J]. Chin Bull Bot, 2013, 48(5): 573-587.
[3] Yanming Miao, Jinzhi Lü, Runcheng Bi, Guiqin Yan. Dynamic Changes of Traits of Leaves from Elaeagnus mollis [J]. Chin Bull Bot, 2012, 47(3): 257-263.
[4] Chenghou Wu, Dongmei Li, Huiqiao Tian. Isolation of Zygotes and Proembryos in Doritis pulcherrima [J]. Chin Bull Bot, 2012, 47(3): 286-291.
[5] Lanxiang Wang, Zhihui Hou, Lixia Hou, Fanggui Zhao, Xin Liu. H2S Induced by H2O2 Mediates Drought-induced Stomatal Closure in Arabidopsis thaliana [J]. Chin Bull Bot, 2012, 47(3): 217-225.
[6] Zhihui Hou, Jing Liu, Lixia Hou, Xidong Li, Xin Liu. H2S May Function Downstream of H2O2 in Jasmonic Acid-induced Stomatal Closure in Vicia faba [J]. Chin Bull Bot, 2011, 46(4): 396-406.
Full text



[1] . [J]. Chin Bull Bot, 1994, 11(专辑): 19 .
[2] Xiao Xiao and Cheng Zhen-qi. Chloroplast 4.5 S ribosomol DNA. II Gene and Origin[J]. Chin Bull Bot, 1985, 3(06): 7 -9 .
[3] CAO Cui-LingLI Sheng-Xiu. Effect of Nitrogen Level on the Photosynthetic Rate, NR Activity and the Contents of Nucleic Acid of Wheat Leaf in the Stage of Reproduction[J]. Chin Bull Bot, 2003, 20(03): 319 -324 .
[4] SONG Li-Ying TAN Zheng GAO Feng DENG Shu-Yan. Advances in in vitro Culture of Cucurbitaceae in China[J]. Chin Bull Bot, 2004, 21(03): 360 -366 .
[5] . [J]. Chin Bull Bot, 1994, 11(专辑): 76 .
[6] LI Jun-De YANG Jian WANG Yu-Fei. Aquatic Plants in the Miocene Shanwang Flora[J]. Chin Bull Bot, 2000, 17(专辑): 261 .
[7] XU Jing-Xian WANG Yu-Fei YANG Jian PU Guang-Rong ZHANG Cui-Fen. Advances in the Research of Tertiary Flora and Climate in Yunnan[J]. Chin Bull Bot, 2000, 17(专辑): 84 -94 .
[8] Sun Zhen-xiao Xia Guang-min Chen Hui-min. Karyotype Analysis of Psathyrostachys juncea[J]. Chin Bull Bot, 1995, 12(01): 56 .
[9] . [J]. Chin Bull Bot, 1994, 11(专辑): 8 -9 .
[10] Yunpu Zheng;Jiancheng Zhao * ;Bingchang Zhang;Lin Li;Yuanming Zhang . Advances on Ecological Studies of Algae and Mosses in Biological Soil Crust[J]. Chin Bull Bot, 2009, 44(03): 371 -378 .