Rooting Induced on in vitro Leaves and Fresh Cuttings of Populus euphratica

doi:10.11983/CBB16024

Abstract

Abstract: Populus euphratica is the unique natural distribution arbor tree growing in the dry desert environment, with root suckering, but cuttings of roots are difficult to grow. This study explored the new ways of rooting induced by Agrobacterium rhizogenes. The strain ATCC11325 can induce hairy roots with no-tropism on in vitro leaf sections. The leaves were cut, scarred, co-cultivated, soaked for 10 min in the microbial agent at OD₆₀₀=0.4 with AS 100 mg·L^-1 added and cultured on tissue culture media for 20 days. Hairy rooting occurred on fresh cuttings soaked with the A. rhizogenes strain solution. The rooting and survival rate reached 60%, and aboveground parts developed more branches and showed vigorous growth. The A. rhizogenes strain promoted rooting of poplar tree fresh cuttings.

Dapeng Wang, Jiaze Tang, Mingcheng Shao, Wenbiao Zhang, Huafang Wang. Rooting Induced on in vitro Leaves and Fresh Cuttings of Populus euphratica[J]. Chinese Bulletin of Botany, 2017, 52(2): 210-217.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB16024

https://www.chinbullbotany.com/EN/Y2017/V52/I2/210

Figures/Tables 5

Table 1 Effect of Agrobacterium rhizogenes ATCC11325 infecting factors on the hairy root induction of Populus euphratica

Factors	Treatment	Leaf discs	Rooting rate (%)
AS (100 mg·L^-1)	Add	30	40.21aA
AS (100 mg·L^-1)	No add	30	5.86 bB
Infection time (min)	5	30	38.54 bB
	10	30	76.87 aA
	20	30	40.21 bB
Scratch	Scratch	30	46.7 aA
Scratch	Unscratch	30	20 bB

Figure 1 PCR and sequencing analyses of Agrobacterium rhizogenes strain ATCC11325(A) MAS gene, lane 1-2: ATCC11325 plasmid DNA; 3-5: Negative control (ddH2O); 6: Marker 3000; (B) rolA gene, lane 1: Marker 3000; 2: Negative control (ddH2O); 3: ATCC11325 plasmid DNA; (C) rolB gene, lane 1: ATCC11325 plasmid DNA; 2-4: Negative control (ddH2O); 5: Marker 3000; (D) rolC gene, lane 1-2: ATCC11325 plasmid DNA; 3-5: Negative control (ddH2O); 6: Marker 3000. Right figures: Similarity of PCR material sequences compared with NCBI accessed gene sequences and their coding amino acid sequences.

Figure 2 Establishment of in vitro culture systems in Populus euphratica(A) Initiation; (B) subculture; (C), (D) Plantlets from shoots

Figure 3 Hairy roots induced from leaf discs of Populus euphratica by Agrobacterium rhizogenes(A) The pre-cultured leaves; (B) Leaves infected 5 min; (C), (D) Leaves infected 10 min; (E) leaves infected 20 min; (F) Scratched and infected leaves

Figure 4 Stock roots and aboveground part from fleshy root cuttings in Populus euphratica infected with Agrobacterium rhizogenes ATCC11325(A) Before cutting; (B) Roots from control; (C) Roots from the cuttings infected with Agrobacterium rhizogenes ATCC11325; (D) Aboveground parts before cutting; (E) Aboveground parts of controls; (F) Aboveground parts of infected cuttings with Agrobacterium rhizogenes ATCC11325

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